Hepatoprotective activity of ethanolic extract of Cissus quadrangularis linn fruits in alcohol-induced liver damage in rats
Abstract
Reactive oxygen species are one of the major reasons for liver damage. The study investigates the hepatoprotective activity of Ethanolic Extract of Cissus Quadrangularis Linn of fruits against ethanol-induced hepatotoxicity. Fruit Powder of Cissus Quadrangularis was successively extracted with ethanol. Preliminary phytochemical tests were done. The hepatoprotective activity of the AST, ALT, ALP, ALB, BIT and BID were assessed in alcohol-induced hepatotoxicity rats. The EECQ showed the presence of alkaloids, carbohydrates, steroids, saponins and triterpenes, while alkaloids, carbohydrates and saponins were present Ethanol produced significant changes in physical (increased liver weight and volume), biochemical (increase in serum alanine transaminase, aspartate transaminase, alkaline phosphatase, direct bilirubin, total bilirubin, cholesterol, triglycerides and decrease in total protein and albumin level). Pre-treatment with EECQ extract significantly prevented the physical, biochemical, histological and functional changes induced by alcohol in the liver. The present study indicates that EECQ extract possessed potential hepatoprotective activity. This activity may be attributed due to the rich phytoconstituents of the plant extract.
Keywords:
Hepatoprotective activity, Cissus Quadrangularis, alcohol-induced hepatotoxicity, Wistar rats, Anti-oxidant studiesDOI
https://doi.org/10.25004/IJPSDR.2021.130306References
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. International Journal of Biochemistry and Cell Biology. 2007; 39(1):44–84.
Mena S, Ortega A, Estrela JM. Oxidative stress in environmental-induced carcinogenesis. Mutation Research. 2009; 674(1-2):36–44.
Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: an overview. African Journal of Microbiology Research. 2009; 3(13):981–996.
McGrath LT, McGleenon BM, Brennan S, McColl D, McIlroy S, Passmore AP. Increased oxidative stress in Alzheimer's disease as assessed with 4-hydroxynonenal but not malondialdehyde. QJM. 2001;94(9): 485–490.
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions. 2006; 160(1):1–40.
Das SK, Vasudevan DM. Alcohol-induced oxidative stress. Life Sciences. 2007; 81(3): 177–187.
Dancygier H, Seitz HK, Mueller S. Alcoholic Liver Disease, Springer, Berlin, Germany, 2010.
Manjunatha BK, Mankani KL, Mukunda SK, Divakara R, Sridar BK, Paul K. “Antioxidant and hepato protective effect of Myristica malabarica seed aril extracts on carbon tetrachloride induced hepatic damage. Global Journal of Biotechnology & Biochemistry. 2011; 6(1):25–30.
Kshirsagar A, Purnima A, Ingawale D, Vyawahare N, Ingale K, Hadambar A. Antioxidant and Hepatoprotective activity of ethanolic extract of Calotropis gigantea against paracetamol induced liver damage in mice. Journal of Cell and Tissue Research. 2009; 9(2):1859–1864.
Babu BH, Shylesh BS, Padikkala J. Antioxidant and hepatoprotective effect of Acanthus ilicifolius. Fitoterapia. 2001; 72(3):272–277.
Jain A, Soni M, Deb L. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. leaves. Journal of Ethnopharmacology. 2008; 115(1):61–66.
Harish R, Shivanandappa T. Antioxidant activity and hepatoprotective potential of Phyllanthus niruri. Food Chemistry. 2006; 95(2):180–185.
Yam MF, Basir R, Asmawi MZ, Rosidah, Ahmad M, Akowuah GA. Antioxidant and hepatoprotective activities of Elephantopus tomentosus ethanol extract. Pharmaceutical Biology. 2008; 46(3):199–206.
Lin CC, Tsai CC, Yen MH. The evaluation of hepatoprotective effects of Taiwan folk medicine “Teng-Khia-U”. Journal of Ethnopharmacology. 1995; 45(2):113–123.
Tsai CC, Lin CC. Anti-inflammatory effects of Taiwan folk medicine “Teng-Khia-U” on carrageenan- and adjuvant-induced paw edema in rats. Journal of Ethnopharmacology. 1998; 64(1):85–89.
Hammer MLA, Johns EA. Tapping an Amazonian plethora: four medicinal plants of Marajo Island, Para (Brazil). Journal of Ethnopharmacology. 1993; 40(1):53–75.
Li YY. 321 cases clinical observation of chronic hepatitis B and liver cirrhosis treated with “Yi Gan Yin”. The Medicine Magazine of China. 2006; 3: 92–93.
Mukherjee Tanmoy, Saha Nabarun, Palbag Satadru. Ethnopharmacology, phytochemistry and pharmacology of ayurvedic plant hadjod - Cissus quadrangularis (L.) International Journal of Research in Ayurveda & Pharmacy. 2016; 7(4):78-83.
Parasuraman S, Kumar EP, Kumar Anil, Emerson SF. Anti-hyperlipidemic effect of Triglize, a polyherbal formulation. International Journal of Pharmacy and Pharmaceutical Sciences. 2010; 2(3): 118-122.
Swamy AHM, Viswanatha, Kulkarni Rucha V, Koti BC, Gadad PC, Thippeswamy AHM, Gore Aparna. Hepatoprotective Effect of Cissus quadrangularis Stem Extract Against Rifampicin-induced Hepatotoxicity in Rats. Indian journal of pharmaceutical sciences. 2012; 74(2): 183-7.
Swamy AHM, Viswanatha, Kulkarni Rucha V, Thippeswamy AHM, Koti BC, Gore Aparna. Evaluation of hepatoprotective activity of Cissus quadrangularis stem extract against isoniazid-induced liver damage in rats. Indian Journal of Pharmacology. 2010; 42(6): 397-400.
Jayan Neethu, Susmita Das, Kumar R, Santhosh, Sabina Ep, Devi S, Asha. Hepatoprotective role of Cissus quadrangularis on lead acetate-induced liver injury in female wistar rats. Asian Journal of Pharmaceutical and Clinical Research. 2017; 10(11): 182-185.
Singh D, Gupta RS. Hepatoprotective Activity of Methanol Extract of Tecomella undulata against Alcohol and Paracetamol induced Hepatotoxicity in rats. Life Sciences and Medicine Research. 2011:26.
Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajas F, Tripathi PC, Seal T, Mukherjee B. Screening of Antioxidant Activity of Three Indian Medicinal Plants Traditionally used for the Management of Neurodegenerative Diseases. J Ethnopharmacol. 84:131-38.
Walker R, Cate W. Liver diseases. Clinical Pharmacy and Therapeutics. Churchill Living Stone: 4th edition; London, 2007, pp. 221-22.
Satyanarayana U, Chalrapani. Liver Function Tests. Fundamentals of Biochemistry Kolkata. 2006:453-58.
Deb AC. Liver Function Tests. Fundamentals of Biochemistry. 8th edition; Kolkata 2008; 6:581.
Published
Abstract Display: 584 
PDF Downloads: 539 
