SIMULTANEOUS ESTIMATION OF DICLOFENAC SODIUM AND RABEPRAZOLE BY HIGH PERFORMANCE LIQIUD CHROMATOGRAPHIC METHOD IN COMBINED DOSAGE FORMS
Abstract
A simple, accurate and reproducible HPLC method have been developed for simultaneous estimation of Diclofenac sodium and rabeprazole from their tablets formulations. A phenomenex C18 (Luna) column of length 250×7.5 mm with particle size of the stationary phase 5 µm and S mobile phase potassium dihydrogen phosphate buffer (pH adjusted to 7.5 with 1 M sodium hydroxide) and acetonitrile in the ratio 60: 40 were used in this study. Retention time was found to be 9.20 min and 6.40 min for Rabeprazole and diclofenac sodium respectively. While that for internal standard as domperidone was 11.87 min at a flow rate of 2ml / min. Linearity was found in the concentration range of 10-50 µg /ml for both the drugs in this method. The results of analysis have been validated statistically and also by recovery studies.
Keywords:
Diclofenac Sodium, Rabeprazole, HPLCDOI
https://doi.org/10.25004/IJPSDR.2009.010110References
Budavari S editors. In The Merck Index. 13th Eds. Merck & Co.Inc. Whitehouse Station (NJ) 2001, 542.
British Pharmacopoeia, Vol II, Deptt. of health and stationary office under the license from the controller of her majesty office for the health minister, UK, 2007, 666.
Budavari S editors. In The Merck Index, 13th Eds. Merck & Co.Inc. Whitehouse Station (NJ) 2001, 1450.
Patil D, Raman B. simultaneous estimation of dextropropoxyphen hydrochloride, diclofenac sodium and paracetamole in capsule by RP HPLC. Indian Drugs 2001; 38: 36-39.
Patel BH , Patel MM , Patel JR, Suhagia, BN. HPLC analysis for simultaneous determination of rabeprazole and domperidone in pharmaceutical formulations. Journal of liquid chromatography & related technologies 2007; 30:439-445.
Sena MM, Choudhary ZF, Collins CH, Poppi RJ. Direct determination of diclofenac in pharmaceutical formulations containing B vitamin by using VU spectrophotometry and partial least square regression. Journal of pharmaceutical and Biomedical Analysis 2004; 36: 734-749.
Chaple DR, Deshpande SS, Dombe MS. Simultaneous spectrophotometric estimation of nimesulide and diclofenac sodium in tablet dosage form. Indian Drugs 2004; 41:108-109.
Rajput SJ, Dobaria NB. Dual spetrophotometric estimation o of rabeprazole sodium and domperidone maleate in their combined pharmaceutical formulations. Indian Drugs 2006; 43:905-908.
Saudagar RB, Saraf S, Saraf S. First order derivative, simultaneous eqation and area under curve method for estimation of domperidone maleate and rabeprazole sodium in tablet dosage form. Indian Drugs 2006; 43:388-392.
Senthie M, Subramanian G, Vasudevan M, Ravisankar S. HPTLC estimation of Tizanidine and diclofenac sodium in combined dosages forms Indian Drugs 2005; 42:465-468.
.Dighe V, Sane, R T, Menon SN, Tambe H N, Lillai S, Gokarn V N. Simultaneous Determination of in diclofenac sodium and paracetamole in pharmaceutical preparation and in bulk drug powder by high performance thin layer chromatography. Journal of planar Chromatography Modern TLC 2006; 19:443-448.
Ramchandran G, Dhorda UJ, Sundersan M. A simultaneous packed coulumn supercritical fliud chromatographic method for chlorzoxazone, diclofenac sodium and acetaminophen bulk and dosages form. Indian Drugs 2001; 38:483-486.
Nerurkar KK, Bhoir IC, Lad NR,Bhagwat AM. Isocratic sepration and simultaneous estimation of three proton pump inhibitor drugs using packed column supercritical fluid chromatography. Indian Drugs 2005; 42:787-791.
Published
Abstract Display: 240 
PDF Downloads: 293 How to Cite
Issue
Section
Copyright (c) 2009 B. Choudhary, A. Goyal, S. L. Khokra, D. Kaushik
This work is licensed under a Creative Commons Attribution 4.0 International License.
