HEPATOPROTECTIVE EFFECT OF WATTAKAKA VOLUBILIS EXTRACT ON ALUMINIUM SULPHATE INDUCED LIVER TOXICITY
Abstract
Wattakaka volubilis (Linn) is used as a phytomedicine for liver diseases. In the present study the effect of methanolic extract of Wattakaka volubilis powder was carried out against the Aluminium toxicity in rat model. Various doses of Wattakaka volubilis extract (100, 200, 400 mg/kg/b.w) was given orally for 30 days to Aluminum sulphate toxicity exposed rats. They were observed each hour for 24 h and each day for 30 days for any changes in behavioural activity and mortality. Clinical observation, body weight, organ weight, biochemical enzymatic analysis and histological examination were carried out. The extract was found to possess hepatoprotective activity in a dose dependent manner and the effect was comparable with silymarin, a standard drug. The extract significantly reduce the toxicity of Aluminium sulphate caused in liver due to the presence of phytoconstituents such as alkaloids, sterols, tannins, triterpenoids and flavonoids which are known hepatoprotectant.
Keywords:
Wattakaka volubilis extract, Aluminium sulphate, liver marker enzymes, histopathologyDOI
https://doi.org/10.25004/IJPSDR.2014.060217References
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4. Domingo JL, Gomez M, Llobet MT, Corbella J. influence of some dietary constituents on aluminium absorption and retention in rats. Kidney Int. 1991; 39:598-601.
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6. Schetinger MRC, Morsch VM, Bohrer D. Aluminium: Interaction with nucleotides and nucleotidases and analytical aspects of 1st determination. Struct bond. 2002; 104:99-137.
7. Osinka E, Kanoniuk D, Kusiak A. Aluminium hemotoxicity mechanisms. Ann Univ Mariae Curie Sklodowska (med) 2004; 59:411-416.
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9. Yokel RA. Brain Uptake, retention and efflux of aluminium and manganese. Co-ordination. Chem Rev. 2002; 228:97-113.
10. Yokel RA, Meurer KA, Skinner TL, Fredenburg AM. The 3-hydroxypyridin -4-ones more effectively chelate aluminium in a rabbit model of aluminium intoxication than does desferrioxamine. Drug metab Dispos. 1996; 24:105-111.
11. Perl DP, Bondy AR. Alzheimer’s disease: X-ray spectrometric evidence of aluminium accumulation in neurofibrillary tangle-bearing neurons. Science 1980; 208:297-299.
12. Good PF, Perl DP, Bierer LM, Schmeidler J. selective accumulation of aluminium and iron in the neurofibrillary tangles of Alzheimer’s disease: a laser microprobe (LAMMA) study. Ann Neurol: 1992; 31:286-292.
13. Bjertness E, Candy .JM, Torvik A, Ince P, Mcarthur F, Taylor GA, et al. Content of brain aluminium is not elevated in Alzheimer disease, Alzheimer Dis Assoc DISORD 1996; 10:171-174.
14. Falen TP. Aluminum as a risk factor in Alzheimer’s disease, with emphasis on drinking water. Brain Res Bull. 2001; 55:187-196.
15. Hirsch EC, Brandel JP, Galle P, Javoyagid F, Agid Y. Iron and a aluminum increase in the substantia nigra of patients with Parkinson’s disease: an X-ray microanalysis. J Neurochem. 1991; 56:446-451.
16. Erasmus RT, Kusnir J, Stevenson WC, Lobo P, Herman MM, Wills MR. Hyperalbuminemia associated with liver transplantation and acute renal failure. Clin transplant. 1995; 9:307-311.
17. The Useful medicinal plants of India. Publication and information Directorate CSIR: New Delhi. India, 1992, pp. 183.
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19. Panda N, Mandal NB, Banerjee S, Sahu NP, Koike K, Nikaidio T, Weber M, Luger, P. Polyhydroxy pregnanes from Dregea volubilis. Phytochemistry. 2003; 61:8400-8405.
20. Sahu NP, Panda N, Mandal NB, Banejee S, Koikea K, Nikadio T. Polyoxy pregnane glycosides from the flowers of Dregea volubilis. Phytochemistry 2002; 61:383-387.
21. Mahato SB, Nandy AK, Roy G. Triterpenoids. Phytochemistry 1992; 31:2199-2205.
22. Biswas M, Mandal, NB, Palit P, Ghosh AK, Bannerjee S, Haldar PK. In vitro anti-leishmanial and anti-tumor activities of a pentacyclic triterpenoid compound isolated from the fruits of Dregea volubilis Benth (Asclepiadaceae). Tropical J Pharm Res. 2009; 8: 127-13.
23. Moumen R, Ait-oukhatar N, Bureau F, Fleury C, Bougle D, Arhan P, et al. Aluminium increases xanthine oxidase activity and disturbs antioxidant status in the rat. J. Trace Elem Med. Biol 2001; 15:89-93.
24. Missel JR, Schetinger MR, Gioda CR, Bohrer DN, Packolski IL, Zanatts N, et al. Chelating effects of novel pyrimidines in a model of aluminium intoxication. J. Inorg Biochem. 2005; 99:1853-1857.
25. Abubakar MG, Taylor A, Fernsga. Aluminium administration is associated with enchanced hepatic oxidant stress that may be offset by dietary vitamin E in the rat. Int. J. Exp. pathol. 2003; 84:45-49.
26. Wilhelm M. Jaeger DE, Schullcablitz H, Hafner D, Idel H. Hepatic perfused rat liver. Toxicol let. 1996; 89:257-263.
27. Gonzalez MA, Alvarez ML, Pisani GB et al. Involvement of oxidative stress in the impairment in biliary secretory function induced by intraperitoneal administration of aluminum to rats. Biol Trace Elem Res. 2007; 116: 329-48.
28. Salvatore F, Sacchetti L, Castaldo G. Multivariate discriminant analysis of biochemical parameters for the differentiation of clinically confounding liver diseases. Clin Chim Acta .1997; 257: 41- 58.
29. Halliwell, B Aeschbach, R, Loliger, J. Arom OI. The characterization of antioxidants. Food Chem. Toxicol. 1995; 33:601-17.
30. Harbone J.B, Baxter, H, Phytochemical dictionary is a hand book of bio active compounds from plant 4 John.st. London; Taylor & Francis. Ltd., 1995.
31. Dawada S, Zade V, Dabhadkar D. Hepatoprotective effect of Wattkaka volubilis extract on paracetamol induced liver toxicity. Int J of Pharmacol Biol Sci. 2012; 6:9-16.
2. Sharma A, Chakraborti KK, Handa SS. Anti-hepatotoxic activity of some Indian herbal formulations as compared to silymarin. Fitoterapia 1991; 62: 229-235
3. Domingo JL, Gomez M, Llobet MT, Corbella J. Parenteral citric acid for aluminium intoxication. Lancet 1988; 2:1362-1363.
4. Domingo JL, Gomez M, Llobet MT, Corbella J. influence of some dietary constituents on aluminium absorption and retention in rats. Kidney Int. 1991; 39:598-601.
5. Schetinger MRC, Boman CD, Morsch VM, Bohrer D, Valentim LM, Rodrigues SR. Effects of aluminium sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice. Bratz J Med Biol Res. 1999; 32:761-766.
6. Schetinger MRC, Morsch VM, Bohrer D. Aluminium: Interaction with nucleotides and nucleotidases and analytical aspects of 1st determination. Struct bond. 2002; 104:99-137.
7. Osinka E, Kanoniuk D, Kusiak A. Aluminium hemotoxicity mechanisms. Ann Univ Mariae Curie Sklodowska (med) 2004; 59:411-416.
8. Williams RJP. What is wrong with aluminium? The JD Birchall Memorial Lecture. J Inorg Biochem. 1999; 76:81-88.
9. Yokel RA. Brain Uptake, retention and efflux of aluminium and manganese. Co-ordination. Chem Rev. 2002; 228:97-113.
10. Yokel RA, Meurer KA, Skinner TL, Fredenburg AM. The 3-hydroxypyridin -4-ones more effectively chelate aluminium in a rabbit model of aluminium intoxication than does desferrioxamine. Drug metab Dispos. 1996; 24:105-111.
11. Perl DP, Bondy AR. Alzheimer’s disease: X-ray spectrometric evidence of aluminium accumulation in neurofibrillary tangle-bearing neurons. Science 1980; 208:297-299.
12. Good PF, Perl DP, Bierer LM, Schmeidler J. selective accumulation of aluminium and iron in the neurofibrillary tangles of Alzheimer’s disease: a laser microprobe (LAMMA) study. Ann Neurol: 1992; 31:286-292.
13. Bjertness E, Candy .JM, Torvik A, Ince P, Mcarthur F, Taylor GA, et al. Content of brain aluminium is not elevated in Alzheimer disease, Alzheimer Dis Assoc DISORD 1996; 10:171-174.
14. Falen TP. Aluminum as a risk factor in Alzheimer’s disease, with emphasis on drinking water. Brain Res Bull. 2001; 55:187-196.
15. Hirsch EC, Brandel JP, Galle P, Javoyagid F, Agid Y. Iron and a aluminum increase in the substantia nigra of patients with Parkinson’s disease: an X-ray microanalysis. J Neurochem. 1991; 56:446-451.
16. Erasmus RT, Kusnir J, Stevenson WC, Lobo P, Herman MM, Wills MR. Hyperalbuminemia associated with liver transplantation and acute renal failure. Clin transplant. 1995; 9:307-311.
17. The Useful medicinal plants of India. Publication and information Directorate CSIR: New Delhi. India, 1992, pp. 183.
18. Panda N, Banerjee S, Mandal NB, Sahu NP. Pregnane glycosides. Nat Prod Comm. 2006; 1:665-669.
19. Panda N, Mandal NB, Banerjee S, Sahu NP, Koike K, Nikaidio T, Weber M, Luger, P. Polyhydroxy pregnanes from Dregea volubilis. Phytochemistry. 2003; 61:8400-8405.
20. Sahu NP, Panda N, Mandal NB, Banejee S, Koikea K, Nikadio T. Polyoxy pregnane glycosides from the flowers of Dregea volubilis. Phytochemistry 2002; 61:383-387.
21. Mahato SB, Nandy AK, Roy G. Triterpenoids. Phytochemistry 1992; 31:2199-2205.
22. Biswas M, Mandal, NB, Palit P, Ghosh AK, Bannerjee S, Haldar PK. In vitro anti-leishmanial and anti-tumor activities of a pentacyclic triterpenoid compound isolated from the fruits of Dregea volubilis Benth (Asclepiadaceae). Tropical J Pharm Res. 2009; 8: 127-13.
23. Moumen R, Ait-oukhatar N, Bureau F, Fleury C, Bougle D, Arhan P, et al. Aluminium increases xanthine oxidase activity and disturbs antioxidant status in the rat. J. Trace Elem Med. Biol 2001; 15:89-93.
24. Missel JR, Schetinger MR, Gioda CR, Bohrer DN, Packolski IL, Zanatts N, et al. Chelating effects of novel pyrimidines in a model of aluminium intoxication. J. Inorg Biochem. 2005; 99:1853-1857.
25. Abubakar MG, Taylor A, Fernsga. Aluminium administration is associated with enchanced hepatic oxidant stress that may be offset by dietary vitamin E in the rat. Int. J. Exp. pathol. 2003; 84:45-49.
26. Wilhelm M. Jaeger DE, Schullcablitz H, Hafner D, Idel H. Hepatic perfused rat liver. Toxicol let. 1996; 89:257-263.
27. Gonzalez MA, Alvarez ML, Pisani GB et al. Involvement of oxidative stress in the impairment in biliary secretory function induced by intraperitoneal administration of aluminum to rats. Biol Trace Elem Res. 2007; 116: 329-48.
28. Salvatore F, Sacchetti L, Castaldo G. Multivariate discriminant analysis of biochemical parameters for the differentiation of clinically confounding liver diseases. Clin Chim Acta .1997; 257: 41- 58.
29. Halliwell, B Aeschbach, R, Loliger, J. Arom OI. The characterization of antioxidants. Food Chem. Toxicol. 1995; 33:601-17.
30. Harbone J.B, Baxter, H, Phytochemical dictionary is a hand book of bio active compounds from plant 4 John.st. London; Taylor & Francis. Ltd., 1995.
31. Dawada S, Zade V, Dabhadkar D. Hepatoprotective effect of Wattkaka volubilis extract on paracetamol induced liver toxicity. Int J of Pharmacol Biol Sci. 2012; 6:9-16.
Published
01-04-2014
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“HEPATOPROTECTIVE EFFECT OF WATTAKAKA VOLUBILIS EXTRACT ON ALUMINIUM SULPHATE INDUCED LIVER TOXICITY”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 2, Apr. 2014, pp. 169-73, https://doi.org/10.25004/IJPSDR.2014.060217.
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How to Cite
“HEPATOPROTECTIVE EFFECT OF WATTAKAKA VOLUBILIS EXTRACT ON ALUMINIUM SULPHATE INDUCED LIVER TOXICITY”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 2, Apr. 2014, pp. 169-73, https://doi.org/10.25004/IJPSDR.2014.060217.
