Anti-colon Cancer Activity of Parkia javanica (Lamk.) Merr. Bark Extract: An In-vitro Study
Abstract
The Methanolic extract of Parkia javanica bark (MEPJB) was examined in vitro to see whether it has any cytotoxic or chemotherapeutic potential against the colon cancer cell lines HCT116 and SW480. Cell viability or proliferation assay and migration assay were performed, which showed significant results. The bark extract exhibited significant cytotoxic potential against both the colon cancer cell lines, and it also inhibited cell migration in a dose-dependent and time-dependent experiment. The IC₅₀ values of MEPJB on the HCT116 cell line for 24 hours, 48 hours, and 72 hours were 83.33 μg/mL, 37.5 μg/mL, and 34.37 μg/mL, respectively, whereas on the SW480 cell line, the IC₅₀ values were 87.5 μg/mL, 40 μg/mL and 31.25 μg/mL for 24 hours, 48 hours, and 72 hours respectively. However, IC₅₀ doses of MEPJB were inactive against the normal, healthy human fibroblast cell line. Cytotoxicity screening of 5-fluorouracil (5-FU), a commonly used drug in colon cancer treatment, was also done on both the colon cancer cell lines HCT116 and SW480, which showed much higher IC₅₀ than MEPJB after 24 hours, 48 hours, and 72 hours of incubation. MEPJB changed the cell morphology and induced apoptosis in colon cancer cell lines, confirmed by a morphological study of treated and untreated colon cancer cell lines and the acridine orange/ethidium bromide (AO/EB) dual staining procedure, respectively. After 48 hours of incubation with respective IC₅₀ concentrations of MEPJB, the treated colon cancer cells showed early and late apoptotic features. So this current research work indicates that the methanolic extract of P. javanica bark has chemotherapeutic potential against colon cancer cells and promotes apoptosis in cancer cells, which results in cell death.
Keywords:
Parkia javanica bark extract, Phenolic compounds, Colon cancer cell lines, Cytotoxic potential, Migration, ApoptosisDOI
https://doi.org/10.25004/IJPSDR.2021.130511References
Qureshi UF, Aslam MN, Ansari MN, Khan M. Role of aspirin as prophylaxis against colorectal cancer. Pakistan Postgraduate Medical Journal. 2018;29(1):16-19.
Swaminathan S, Katoch VM. Consolidated Report of Hospital Based Cancer Registries 2012-2014. Bengaluru: Indian Council of Medical Research. 2016.
Rawla P, Sunkara T, Barsouk A. Epidemiology of colorectal cancer: incidence, mortality, survival, and risk factors. Przeglad gastroenterologiczny. 2019;14(2):89.
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians. 2018;68(6):394-424.
Ferlay J, Ervik M, Lam F, Colombet M, Mery L, Piñeros M, Znaor A, Soerjomataram I, Bray F. Global cancer observatory: cancer today. Lyon, France: international agency for research on cancer. 2018;17:1-6.
Best L, Simmonds P, Baughan C, Buchanan R, Davis C, Fentiman I, George S, Gosney M, Northover J, Williams C. Palliative chemotherapy for advanced or metastatic colorectal cancer. Cochrane Database of Systematic Reviews. 2000(1).
Goldberg RM, Sargent DJ, Morton RF, Fuchs CS, Ramanathan RK, Williamson SK, Findlay BP, Pitot HC, Alberts SR. A randomized controlled trial of fluorouracil plus leucovorin, irinotecan, and oxaliplatin combinations in patients with previously untreated metastatic colorectal cancer. Journal of Clinical Oncology. 2004;22(1):23-30.
de Gramont AD, Figer A, Seymour M, Homerin M, Hmissi A, Cassidy J, Boni C, Cortes-Funes H, Cervantes A, Freyer G, Papamichael D. Leucovorin and fluorouracil with or without oxaliplatin as first-line treatment in advanced colorectal cancer. Journal of Clinical Oncology. 2000;18(16):2938-2947.
Douillard J, Cunningham D, Roth AD, Navarro M, James RD, Karasek P, Jandik P, Iveson T, Carmichael J, Alakl M, Gruia G. Irinotecan combined with fluorouracil compared with fluorouracil alone as first-line treatment for metastatic colorectal cancer: a multicentre randomised trial. The Lancet. 2000;355(9209):1041-1047.
Somkuwar AP. Studies on anticancer effects of Ocimum sanctum and Withania somnifera on experimentally induced cancer in mice, Doctoral dissertation, Jawaharlal Nehru Krishi Viswavidyalaya, Jabalpur, India, 2003.
Govind P, Madhuri S. Medicinal plants: better remedy for neoplasm. Indian drugs. 2006; 43(11):869-74.
Macdonald JS. Toxicity of 5-fluorouracil. Oncology (Williston Park, NY). 1999;13(7 Suppl 3):33-34.
Rexroth G, Scotland VJ. Cardiac toxicity of 5-fluorouracil. Medizinische Klinik (Munich, Germany: 1983). 1994;89(12):680-688.
Rastogi N, Chag M, Ayyagari S. Myocardial ischemia af ter 5-fluorouracil chemotherapy. International journal of cardiology. 1993;42(3):285-287.
Ochwang’i DO, Kimwele CN, Oduma JA, Gathumbi PK, Mbaria JM, Kiama SG. Medicinal plants used in treatment and management of cancer in Kakamega County, Kenya. Journal of Ethnopharmacology. 2014;151(3):1040-1055.
Freiburghaus F, Kaminsky R, Nkunya MH, Brun R. Evaluation of African medicinal plants for their in vitro trypanocidal activity. Journal of ethnopharmacology. 1996;55(1):1-11.
Costa-Lotufo LV, Khan MT, Ather A, Wilke DV, Jimenez PC, Pessoa C, de Moraes ME, de Moraes MO. Studies of the anticancer potential of plants used in Bangladeshi folk medicine. Journal of Ethnopharmacology. 2005;99(1):21-30.
Cai YZ, Sun M, Xing J, Luo Q, Corke H. Structure–radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life sciences. 2006;78(25):2872-2888.
Fouché G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ, Senabe J. In vitro anticancer screening of South African plants. Journal of ethnopharmacology. 2008; 119(3):455-461.
Kamatou GP, Van Zyl RL, Davids H, Van Heerden FR, Lourens AC, Viljoen AM. Antimalarial and anticancer activities of selected South African Salvia species and isolated compounds from S. radula. South African Journal of Botany. 2008;74(2):238-243.
Sinha SC. Medicinal plants of Manipur. Manipur Cultural Integration Conference, Imphal, 1996.
Majumdar K, Datta BK, Roy D. Inventory and status of medicinal trees of Tripura. Indian Medicinal Plants. Aavishkar Publishers: Jaipur, India, 2009:93-123.
Bhardwaj S., Gakhar SK. Ethnomedicinal plants used by the tribals of Mizoram to cure cuts & wounds. Indian J. Traditional knowledge. 2005;4(1):75- 80.
Patra K, Jana S, Sarkar A, Karmakar S, Jana J, Gupta M, Mukherjee G, De UC, Mandal DP, Bhattacharjee S. Parkia javanica extract induces apoptosis in S-180 cells via the intrinsic pathway of apoptosis. Nutrition and cancer. 2016;68(4):689-707.
Chanu KV, Devi LG, Srivastava SK, Thakuria D, Kataria M and Telang AG. Phytochemical analysis and evaluation of anticancer activity of Parkia javanica seeds. The Pharma Innovation Journal. 2018;7(5):305-311.
Horbone, J.B., In: Phytochemical methods, 2nd edition. Chapman and Hall, New York,1984.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods. 1983;65(1-2): 55-63.
Ebeling S, Naumann K, Pollok S, Wardecki T, Vidal-y-Sy S, Nascimento JM, Boerries M, Schmidt G, Brandner JM, Merfort I. From a traditional medicinal plant to a rational drug: understanding the clinically proven wound healing efficacy of birch bark extract. Plos one. 2014;9(1):e86147.
Krishnamoorthy JR, Sumitira S, Ranjith MS, Gokulshankar S, Ranganathan S, Mohanty BK, Prabhakaran G. An in vitro study of wound healing effect of a poly-herbal formulation as evidenced by enhanced cell proliferation and cell migration. Egypt. Dermatol. Online J. 2012;8(1):1-7.
Toné S, Sugimoto K, Tanda K, Suda T, Uehira K, Kanouchi H, Samejima K, Minatogawa Y, Earnshaw WC. Three distinct stages of apoptotic nuclear condensation revealed by time-lapse imaging, biochemical and electron microscopy analysis of cell-free apoptosis. Experimental cell research. 2007;313(16):3635-3644.
Liu K, Liu PC, Liu R, Wu X. Dual AO/EB staining to detect apoptosis in osteosarcoma cells compared with flow cytometry. Medical science monitor basic research. 2015;21:15-20.
Maiti G. Plant diversity of India and its conservation, utilization with special reference to N. E. region. In: Proc. Sem. Biodiversity, Tripura University, India, 2004, pp. 6.
Tripathi RS. and Barik SK. National Biodiversity Strategy and Action Plan Report for Northeast India. Ministry of Environment and Forests, New Delhi, India, 2003.
Chatterjee S, Saikia A, Dutta P, Ghosh D, Pangging G, Goswami AK. Biodiversity significance of North east India. WWF-India, New Delhi, 2006, pp. 1-71.
Deb DB. The flora of Tripura state. Today & Tomorrow’s Printers and Publishers, 1983.
Das H B, Majumdar K, Datta BK, Roy D. Ethnobotanical uses of some plants by Tripuri and Reang tribes of Tripura. Natural Product Radiance. 2009;8(2):172- 180.
Roy M, Chakama B, Datta BK, Gupta AK. Traditional knowledge of medicinal plants used by Chakma tribal community of Tripura, India. Pleione. 2010;4(1):105-112.
Saha S, Karmakar P, Sil SK. Antibacterial activities of Parkia javanica extract against multidrug resistant gram negative bacteria predominantly found in skin wound. Int J Pharm Bio Sci. 2018;8(1):96-102.
Sil SK, Saha S, Karmakar P. Reactive oxygen species as possible mediator of antibacterial activity of Parkia javanica, against bacterial species predominantly found in chronic wound. Journal of Drug Delivery and Therapeutics. 2018;8(1):43-47.
Saha S, Basu Mullick J, Ray Choudhury P, Saha P, Chakraborty D, Sil SK. Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains. Int. J. Curr. Microbiol. App. Sci. 2016;5(8):538-544.
Mullick JB, Majumdar T, Reddy KV, Mukherjee S, Sil SK. Activity of the medicinal plant Parkia Javanica against multidrug-resistant Neisseria gonorrhoeae and other clinical isolates. Asian J. Pharm. Clin. Res. 2019;12:83-6.
Saha R, Basu JM, Mookerjee A, Mohanta BC, Chaudhuri SK, Roy S, Dinda B, Sil SK. In vitro and in vivo studies on antiproliferative activity of Parkia javanica. Journal of Applied Bioscience. 2011;37(2):131-6.
Liang CC, Park AY, Guan JL. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature protocols. 2007;2(2):329-333.
Yarrow JC, Perlman ZE, Westwood NJ, Mitchison TJ. A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods. BMC Biotechnol. 2004;4:21.
Gulhati P, Bowen KA, Liu J, Stevens PD, Rychahou PG, Chen M, Lee EY, Weiss HL, O'Connor KL, Gao T, Evers BM. mTORC1 and mTORC2 regulate EMT, motility, and metastasis of colorectal cancer via RhoA and Rac1 signaling pathways. Cancer research. 2011;71(9):3246-3256.
Fisher DE. Apoptosis in cancer therapy: crossing the threshold. Cell. 1994;78(4):539-542.
Yi JM, Kim MS, Lee EH, Wi DH, Lee JK, Cho KH, Hong SH, Kim HM. Induction of apoptosis by Paljin-Hangahmdan on human leukemia cells. Journal of ethnopharmacology. 2003;88(1):79-83.
Nandi S, Vracko M, Bagchi MC. Anticancer activity of selected phenolic compounds: QSAR studies using ridge regression and neural networks. Chemical biology & drug design. 2007;70(5):424-436.
Boyer N, Morrison KC, Kim J, Hergenrother PJ, Movassaghi M. Synthesis and anticancer activity of epipolythiodiketopiperazine alkaloids. Chemical science. 2013;4(4):1646-1657.
Srivastava V, Negi AS, Kumar JK, Gupta MM, Khanuja SP. Plant-based anticancer molecules: a chemical and biological profile of some important leads. Bioorganic & medicinal chemistry. 2005;13(21):5892-5908.
Boreddy SR, Srivastava SK. Pancreatic cancer chemoprevention by phytochemicals. Cancer letters. 2013;334(1):86-94.
Vijayababu MR, Arunkumar A, Kanagaraj P, Venkataraman P, Krishnamoorthy G, Arunakaran J. Quercetin downregulates matrix metalloproteinases 2 and 9 proteins expression in prostate cancer cells (PC-3). Molecular and cellular biochemistry. 2006;287(1):109-116.p
Yildirim I, Kutlu T. Anticancer agents: saponin and tannin. International journal of biological chemistry. 2015;9(6):332-340.
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