ENTRAPMENT OF SEED EXTRACT OF NIGELLA SATIVA INTO THERMOSENSITIVE (NIPAAM–CO–VP) CO-POLYMERIC MICELLES AND ITS ANTIBACTERIAL ACTIVITY
Abstract
Thermosensitive N-Isopropyl acryl amide-N-Vinyl 2-pyrollidone (NIPAAm-VP) co-polymeric micelle synthesized by radical copolymerization and the extract of Nigella sativa is entrapped in this polymeric system to check the release of the bioactive compound and evaluated its antibacterial activity. The size of nanoparticles was measured 75-110 nm and 1 % drug is entrapped. In this work, the seed extract has been loaded into the polymeric micelle and its effectiveness has been evaluated against gram positive strain of Staphylococcus aureus. Bacillus subtilis and a gram negative strain of Escherichia coli. Nigella sativa loaded polymeric micelles found hundred times efficient in comparison of the naked one. Drug release from the polymeric micellar system at 37-42oC in different time intervals i.e. 30 min - 4 weeks is measured 7.14-66.66%. The above findings revealed that this thermosensitive polymeric system would more effectively release the drug into the body when infectious states are functional i.e. higher temperature and may have possibility to use as a novel drug delivery system for more herbal drugs for patient compliance.
Keywords:
NIPAAm-VP co-polymeric nanoparticles, Thermosensitive micelles, Nigella sativa, Staphylococcus aureus, Bacillus subtilis antibacterial activity.DOI
https://doi.org/10.25004/IJPSDR.2011.030317References
1. Collin CH, Lyne PM and Grange JM Microbiological Methods, 7th ed., Butter Worth-Heinemann, Linacre House, Oxford, U. K. 1995.
2. Salman MT, Khan RA, Shukla I. Antimicrobial activity of Nigella sativa Linn. Seed oil against multi-drug resistant bacteria from clinical isolates. Natural Product Radiance 2008; 7: 10-14.
3. Al-Ghamdi MS. The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa. Journal of Ethnopharmacology 2001; 76: 45-48.
4. Basha LA, Rashed MS, Aboul-Enein HY. TLC Assay of Thymoquinone in Black Seed Oil (Nigella sativa Linn) and Identification of Dithymoquinone and Thymol. Journal of Liquid Chromatography & Related Technologies 1995; 18: 105-115.
5. Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytotherapy Research 2003; 17: 299-305.
6. Toama MA, El-Alfy TS, El-Fatatry HM. Antimicrobial Activity of the Volatile Oil of Nigella sativa Linneaus Seeds. Antimicrobial Agents and Chemotherapy 1974; 6: 225-226.
7. Arici M, Sagdic O, Gecgel U. Antibacterial effect of Turkish black cumin (Nigella sativa L.) oils. Grasas y Aceites. 2005; 56: 259-262.
8. Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research 2000; 14: 323-328.
9. Hosseinzadeh H, Bazzaz B, Haghi MM. Antibacterial Activity of Total Extracts and Essential oil of Nigella sativa L. Seeds in Mice. Pharmacolgyonline 2007; 2: 429-435.
10. Mashhadian N, Rakhshandeh H. Antibacterial and antifungal effects of Nigella sativa extracts against S. aureus, P. aeroginosa and C. albicans. Pak J Med Sci. 2005; 21: 47-52.
11. Toppozada HH, Mazloum HA. The antibacterial properties of the Nigella sativa l. seeds. Active principle with some clinical applications. J Egypt Med Assoc. 1965; 48: 187-202.
12. Hanafy MS, Hatem ME. Studies on the antimicrobial activity of Nigella sativa seed (black cumin). J Ethnopharmacol. 1991; 34: 275-278.
13. Zaher KS, Ahmed WM, Zerizer SN. Observations on the Biological Effects of Black Cumin Seed (Nigella sativa) and Green Tea (Camellia sinensis). Global Veterinaria. 2008; 2: 198-204.
14. El-Baroty GS, Abd El-Baky HH, Farag RS, Saleh MA. Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils. African Journal of Biochemistry Research 2010; 4(6): 167-174.
15. Mohamed E, Naglaa IM, Abdel HM. Nigella sativa L. oil protects against induced hepatotoxicity and improves serum lipid profile in rats. Arzneimittelforschung. 2000; 50: 832-836.
16. Padhye S, Banerjee S, Ahmad A, Mohammad R, Fazlul H. From here to eternity-the secret of Pharaohs: Therapeutic potential of black cumin seeds and beyond. Cancer Ther. 2008; 6: 495-510.
17. Khanam M, Dewan ZF. Effects of the crude and the n-hexane extract of Nigella sativa Linn. (kalajira) upon diabetic rats. Bangladesh J. of Pharmacol. 2008; 4: 17-20.
18. Bisht S, Feldmann G, Soni S, Ravi R, Karikar C, Maitra A. Polymeric nanoparticle-encapsulated curcumin ("nanocurcumin"):a novel strategy for human cancer therapy, Journal of Nanobiotechnology 2007; 5: 1-18.
19. Van Vlerken LE, Amiji MM. Multi-functional polymeric nanoparticles for tumour-targeted drug delivery. Expert Opinion Drug Deliv. 2006; 3: 205-16.
20. Avgoustakis K. Pegylated poly (lactide) and poly (lactide-co-glycolide) nanoparticles: preparation, properties and possible application in drug delivery. Current Drug Delivery 2004; 1: 321–333.
21. Ghosh PK, Murthy SR. Microemulsions: a potential drug delivery system. Curr. Drug Deliv. 2006; 3: 167-180.
22. Torchilin VP. Structure and design of polymeric surfactant-based drug delivery systems. J Control Release 2001; 73: 137-172.
23. Cicek H, Tuncel A. Immobilization of α-chymotrypsin in thermally reversible isopropylacrylamide copolymer gel. J. Polym. Sci, Part A Polym Chem. 1998; 36: 543.
24. Si-Shen F. Nanoparticles of biodegradable polymers for new-concept chemotherapy. Expert Review of Medical Devices 2004; 1: 115-125.
25. Song M, Guo D, Pan C, Jiang H, Chen C, Zhang R, Gu Z, Wang X. The application of poly (N-isopropylacrylamide)-co-polystyrene nanofibers as an additive agent to facilitate the cellular uptake of an anticancer drug. Nanotechnology 2008; 19: 102-165.
26. Yoshida R, Kaneko Y, Sakai K. Positive thermosensitive pulsatile drug release using negative thermosensitive hydrogels. Journal of Controlled Release 1994; 32: 97-102.
27. Na K, Lee KH, Lee DH. Biodegradable thermo-sensitive nanoparticles from poly (L-lactic acid)/poly (ethylene glycol) alternating multiblock copolymer for potential anti-cancer drug carrier. Eur J Pharm Sci. 2006; 27: 115–22.
28. Hennink WE, Van Nostrum CF. Novel crosslinking method to design hydrogels. Advanced drug delivery review 2002; 54: 1, 13.
29. Wei H, Zheng XZ, Zhou Y, Cheng SX, Zhuo RX. Self-assembled thermoresponsive micelles of poly (N-isopropylacrylamide-b-methyl methacrylate). Biomaterials 2006; 27(9): 2028-2034.
30. Ichikawa H, Fukumori YA. Novel positively thermosensitive controlled release microcapsule with membrane of nano-sized poly (N-isopropylacrylamie) gel ispesed in ethylcellulose matrix. J. Controlled Release 2000; 63: 107-11.
31. Maitra A, Gaur U, Ghosh PC, Maitra AN. Tumour Targeted Delivery of Encapsulated Dextran-Doxorubicin Conjugate Using Chitosan Nanoparticles as Carrier. Journal of Controlled Release 2001; 74: 317-323.
32. Wu XY, Lee PI. Preparation and characterization of thermal and pH sensitive nanospheres. Pharm. Res. 1993; 10: 1544-47.
33. Rapoport N. Physical stimuli-responsive polymeric micelles for anti-cancer drug delivery. Progress in Polymer Science 2007; 32(8-9): 962-990.
34. Chavanpatil MD, Patil Y, Panyam J. Susceptibility of nanoparticle-encapsulated paclitaxel to p-glycoprotein-mediated drug efflux. Int. J. Pharm. 2006; 320: 150–156.
35. Verma AK, Chanchal A, Maitra A. Co-polymeric hydrophilic nanospheres for drug delivery: Release kinetics and cellular uptake. Indian Journal of Experiment Biology 2010; 48: 1043-1052.
36. Bauer AW, Kirby WMM, Sherris JC, Turk M. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 1966; 45:493-496
37. Pelczar MJ, Chan ECS, Krieg NR. Microbiology Concepts and Applications, 6th ed., McGraw-Hill Inc., New York, U.S.A. 1999.
2. Salman MT, Khan RA, Shukla I. Antimicrobial activity of Nigella sativa Linn. Seed oil against multi-drug resistant bacteria from clinical isolates. Natural Product Radiance 2008; 7: 10-14.
3. Al-Ghamdi MS. The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa. Journal of Ethnopharmacology 2001; 76: 45-48.
4. Basha LA, Rashed MS, Aboul-Enein HY. TLC Assay of Thymoquinone in Black Seed Oil (Nigella sativa Linn) and Identification of Dithymoquinone and Thymol. Journal of Liquid Chromatography & Related Technologies 1995; 18: 105-115.
5. Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytotherapy Research 2003; 17: 299-305.
6. Toama MA, El-Alfy TS, El-Fatatry HM. Antimicrobial Activity of the Volatile Oil of Nigella sativa Linneaus Seeds. Antimicrobial Agents and Chemotherapy 1974; 6: 225-226.
7. Arici M, Sagdic O, Gecgel U. Antibacterial effect of Turkish black cumin (Nigella sativa L.) oils. Grasas y Aceites. 2005; 56: 259-262.
8. Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research 2000; 14: 323-328.
9. Hosseinzadeh H, Bazzaz B, Haghi MM. Antibacterial Activity of Total Extracts and Essential oil of Nigella sativa L. Seeds in Mice. Pharmacolgyonline 2007; 2: 429-435.
10. Mashhadian N, Rakhshandeh H. Antibacterial and antifungal effects of Nigella sativa extracts against S. aureus, P. aeroginosa and C. albicans. Pak J Med Sci. 2005; 21: 47-52.
11. Toppozada HH, Mazloum HA. The antibacterial properties of the Nigella sativa l. seeds. Active principle with some clinical applications. J Egypt Med Assoc. 1965; 48: 187-202.
12. Hanafy MS, Hatem ME. Studies on the antimicrobial activity of Nigella sativa seed (black cumin). J Ethnopharmacol. 1991; 34: 275-278.
13. Zaher KS, Ahmed WM, Zerizer SN. Observations on the Biological Effects of Black Cumin Seed (Nigella sativa) and Green Tea (Camellia sinensis). Global Veterinaria. 2008; 2: 198-204.
14. El-Baroty GS, Abd El-Baky HH, Farag RS, Saleh MA. Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils. African Journal of Biochemistry Research 2010; 4(6): 167-174.
15. Mohamed E, Naglaa IM, Abdel HM. Nigella sativa L. oil protects against induced hepatotoxicity and improves serum lipid profile in rats. Arzneimittelforschung. 2000; 50: 832-836.
16. Padhye S, Banerjee S, Ahmad A, Mohammad R, Fazlul H. From here to eternity-the secret of Pharaohs: Therapeutic potential of black cumin seeds and beyond. Cancer Ther. 2008; 6: 495-510.
17. Khanam M, Dewan ZF. Effects of the crude and the n-hexane extract of Nigella sativa Linn. (kalajira) upon diabetic rats. Bangladesh J. of Pharmacol. 2008; 4: 17-20.
18. Bisht S, Feldmann G, Soni S, Ravi R, Karikar C, Maitra A. Polymeric nanoparticle-encapsulated curcumin ("nanocurcumin"):a novel strategy for human cancer therapy, Journal of Nanobiotechnology 2007; 5: 1-18.
19. Van Vlerken LE, Amiji MM. Multi-functional polymeric nanoparticles for tumour-targeted drug delivery. Expert Opinion Drug Deliv. 2006; 3: 205-16.
20. Avgoustakis K. Pegylated poly (lactide) and poly (lactide-co-glycolide) nanoparticles: preparation, properties and possible application in drug delivery. Current Drug Delivery 2004; 1: 321–333.
21. Ghosh PK, Murthy SR. Microemulsions: a potential drug delivery system. Curr. Drug Deliv. 2006; 3: 167-180.
22. Torchilin VP. Structure and design of polymeric surfactant-based drug delivery systems. J Control Release 2001; 73: 137-172.
23. Cicek H, Tuncel A. Immobilization of α-chymotrypsin in thermally reversible isopropylacrylamide copolymer gel. J. Polym. Sci, Part A Polym Chem. 1998; 36: 543.
24. Si-Shen F. Nanoparticles of biodegradable polymers for new-concept chemotherapy. Expert Review of Medical Devices 2004; 1: 115-125.
25. Song M, Guo D, Pan C, Jiang H, Chen C, Zhang R, Gu Z, Wang X. The application of poly (N-isopropylacrylamide)-co-polystyrene nanofibers as an additive agent to facilitate the cellular uptake of an anticancer drug. Nanotechnology 2008; 19: 102-165.
26. Yoshida R, Kaneko Y, Sakai K. Positive thermosensitive pulsatile drug release using negative thermosensitive hydrogels. Journal of Controlled Release 1994; 32: 97-102.
27. Na K, Lee KH, Lee DH. Biodegradable thermo-sensitive nanoparticles from poly (L-lactic acid)/poly (ethylene glycol) alternating multiblock copolymer for potential anti-cancer drug carrier. Eur J Pharm Sci. 2006; 27: 115–22.
28. Hennink WE, Van Nostrum CF. Novel crosslinking method to design hydrogels. Advanced drug delivery review 2002; 54: 1, 13.
29. Wei H, Zheng XZ, Zhou Y, Cheng SX, Zhuo RX. Self-assembled thermoresponsive micelles of poly (N-isopropylacrylamide-b-methyl methacrylate). Biomaterials 2006; 27(9): 2028-2034.
30. Ichikawa H, Fukumori YA. Novel positively thermosensitive controlled release microcapsule with membrane of nano-sized poly (N-isopropylacrylamie) gel ispesed in ethylcellulose matrix. J. Controlled Release 2000; 63: 107-11.
31. Maitra A, Gaur U, Ghosh PC, Maitra AN. Tumour Targeted Delivery of Encapsulated Dextran-Doxorubicin Conjugate Using Chitosan Nanoparticles as Carrier. Journal of Controlled Release 2001; 74: 317-323.
32. Wu XY, Lee PI. Preparation and characterization of thermal and pH sensitive nanospheres. Pharm. Res. 1993; 10: 1544-47.
33. Rapoport N. Physical stimuli-responsive polymeric micelles for anti-cancer drug delivery. Progress in Polymer Science 2007; 32(8-9): 962-990.
34. Chavanpatil MD, Patil Y, Panyam J. Susceptibility of nanoparticle-encapsulated paclitaxel to p-glycoprotein-mediated drug efflux. Int. J. Pharm. 2006; 320: 150–156.
35. Verma AK, Chanchal A, Maitra A. Co-polymeric hydrophilic nanospheres for drug delivery: Release kinetics and cellular uptake. Indian Journal of Experiment Biology 2010; 48: 1043-1052.
36. Bauer AW, Kirby WMM, Sherris JC, Turk M. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 1966; 45:493-496
37. Pelczar MJ, Chan ECS, Krieg NR. Microbiology Concepts and Applications, 6th ed., McGraw-Hill Inc., New York, U.S.A. 1999.
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01-07-2011
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“ENTRAPMENT OF SEED EXTRACT OF NIGELLA SATIVA INTO THERMOSENSITIVE (NIPAAM–CO–VP) CO-POLYMERIC MICELLES AND ITS ANTIBACTERIAL ACTIVITY”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 3, July 2011, pp. 246-52, https://doi.org/10.25004/IJPSDR.2011.030317.
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How to Cite
“ENTRAPMENT OF SEED EXTRACT OF NIGELLA SATIVA INTO THERMOSENSITIVE (NIPAAM–CO–VP) CO-POLYMERIC MICELLES AND ITS ANTIBACTERIAL ACTIVITY”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 3, July 2011, pp. 246-52, https://doi.org/10.25004/IJPSDR.2011.030317.
