EVALUATION OF PROTECTIVE EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF FRUIT PEELS OF PUNICA GRANATUM LINN AGAINST ULCERATIVE COLITIS IN RATS
Abstract
The study was designed to evaluate the protective effect of hydro-alcoholic extract of fruits peels of Punica granatum Linn. against acetic acid and trinitrobenzene sulfonic acid (TNBS) induced ulcerative colitis in rats. Acute oral toxicity study was performed to find out the test dose according to OECD guidelines 425 and hydro-alcoholic extract of P. granatum fruit peel (PGPE) was found to be safe at a dose of 2000 mg/kg body weight. The animals pretreated with hydro-alcoholic extract of P. granatum fruit peel (200 mg/kg and 400 mg/kg respectively) significantly restored the altered hematological, biochemical parameters to normal levels when compared with control. The protective effect of P. granatum peel extract was comparable to standard sulfasalazine. The findings of the present study revealed that the hydro-alcoholic extract of P. granatum fruit peel possessed a dose dependent significant inhibitory activity against ulcerative colitis. The results obtained established the efficacy of the Punica granatum fruit peel against inflammatory bowel diseases possibly by its anti-inflammatory and antioxidant properties.
Keywords:
Acetic acid, Punica granatum, Sulfasalazine, TNBS, Ulcerative colitisDOI
https://doi.org/10.25004/IJPSDR.2014.060308References
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18. Das S, Lalit K. Effect of ethanolic extract of leaves of Moringa olifera lam. on acetic acid induced colitis in albino rats. Asian J Pharm Clin Res. 2012; 5(3):110-4.
19. Stefan W, Markus F. Mouse models of inflammatory bowel disease. Adv Drug Deliv Rev. 2007; 59:1073-83.
20. Sartor RB. Pathogenesis and immune mechanisms of chronic inflammatory bowel disease. The Am J Gastroenterol. 1997; 92:5S-11S.
21. Zama D, Meraihi Z, Tebibel S, Benayssa W, Benayache F, Benayache S et al. Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L. Indian J Pharmacol. 2007; 39(3):145-50.
22. Visavadiya NP, Narasimhacharya AVRL. Hypolipidemic and antioxidant activities of Asparagus racemosus in hypercholesteremic rats. Indian J Pharmacol 2005; 37(6):376-80.
23. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta 1979; 582(1):67-78.
24. Joshi SV, Vyas BA, Shah PD, Shah DR, Shah SA, Gandhi TR. Protective effect of aqueous extract of Oroxylum indicum Linn. (Root bark) against DNBS-induced colitis in rats. Indian J Pharmacol. 2011; 43(6):656-61.
2. Stefan W, Markus N. Mouse models of inflammatory bowel disease. Adv Drug Deliv Rev. 2007; 59:1073-83.
3. Miyako M, Noboru H. Protective effect of epigallocatechin gallate on acute experimental colitis. J Health Sci. 2005; 51(3):362-4.
4. Schmidt C, Stallimach A. Etiology and pathogenesis of inflammatory bowel disease. Minerva Gastroenterol Dietol. 2005; 51:127-45.
5. Sandborn W, Yednock T. Novel approaches to treating inflammatory bowel disease targeting alpha-4 integrin. Am J Gastroenterol. 2003; 98:2372-82.
6. Devlin HB, Datta D, Dellipiani AW. The incidence and prevalence of inflammatory bowel disease in North Tees Health District. World J Surg. 1980; 4:183-93.
7. Kamm MA, Senapati A. Drug management of ulcerative colitis. BMJ 1992; 305:35-9.
8. Shanahan F. Inflammatory bowel disease: immunodiagnostics, immunotherapeutics and ecotherapeutics. Gastroenterol. 2001; 20:622-35.
9. Noda Y, Kaneyuki T, Mori A, Packer L. Antioxidant activities of pomegranate fruit extract and it’s anthocyanidins: delphinidin, cyanidin, and pelargonidin. J Agric Food Chem. 2002:50:166-71.
10. Trease GE, Evans MC. Text book of Pharmacognosy. 1st ed. London: BaillaireTindall; 1983, 12, pp. 193-336.
11. OECD/OCDE. 425 OECD guidelines for testing of chemicals acute oral toxicity, up and down procedure, 2001; 26:1-26.
12. Murat Z, Mustafa K, Erhan A, Ozgur F, Murat A, Gökhan İ. The comparative effects of calcium channel blockers in an experimental colitis model in rats. Turk J Gastroenterol. 2004; 15:266-99.
13. Stefan W, Markus F. Mouse models of inflammatory bowel disease. Adv Drug Deliv Rev 2007; 59:1073-83.
14. Balaraman R, Bafna PA, Kolhapure SA. Antioxidant activity of DHC-1* a herbal formulation. J Ethnopharmacol. 2004; 94:135-41.
15. Oyaizu M. Studies on product of browing reactions preparations from glucoseamine. Jap J Nutrition 1986; 44:307-9.
16. Oyedemi SO, Bradley G, Afolaya AJ. In-vitro and In-vivo antioxidant activities of aqueous extract of Strychnos henningsii Gilg. African J Pharm Pharmacol. 2010; 4(2):70-8.
17. Luna LG. Manual of histology and staining methods of armed forces institute of Pathology. 3rd ed. New York: Mc Graw Hill Book Co, 1998, pp. 258.
18. Das S, Lalit K. Effect of ethanolic extract of leaves of Moringa olifera lam. on acetic acid induced colitis in albino rats. Asian J Pharm Clin Res. 2012; 5(3):110-4.
19. Stefan W, Markus F. Mouse models of inflammatory bowel disease. Adv Drug Deliv Rev. 2007; 59:1073-83.
20. Sartor RB. Pathogenesis and immune mechanisms of chronic inflammatory bowel disease. The Am J Gastroenterol. 1997; 92:5S-11S.
21. Zama D, Meraihi Z, Tebibel S, Benayssa W, Benayache F, Benayache S et al. Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L. Indian J Pharmacol. 2007; 39(3):145-50.
22. Visavadiya NP, Narasimhacharya AVRL. Hypolipidemic and antioxidant activities of Asparagus racemosus in hypercholesteremic rats. Indian J Pharmacol 2005; 37(6):376-80.
23. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta 1979; 582(1):67-78.
24. Joshi SV, Vyas BA, Shah PD, Shah DR, Shah SA, Gandhi TR. Protective effect of aqueous extract of Oroxylum indicum Linn. (Root bark) against DNBS-induced colitis in rats. Indian J Pharmacol. 2011; 43(6):656-61.
Published
01-07-2014
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“EVALUATION OF PROTECTIVE EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF FRUIT PEELS OF PUNICA GRANATUM LINN AGAINST ULCERATIVE COLITIS IN RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 3, July 2014, pp. 211-5, https://doi.org/10.25004/IJPSDR.2014.060308.
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How to Cite
“EVALUATION OF PROTECTIVE EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF FRUIT PEELS OF PUNICA GRANATUM LINN AGAINST ULCERATIVE COLITIS IN RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 3, July 2014, pp. 211-5, https://doi.org/10.25004/IJPSDR.2014.060308.
