COMPARATIVE IN-VITRO ANTIOXIDANT SCREENING OF METHANOLIC EXTRACT OF COSTUS PICTUS & ITS SILVER NANOPARTICLES
Abstract
To compare in-vitro antioxidant activity of Methanolic extract of Costus pictus (MECP) and its Silver nanoparticles (MECPAgNPs) by various methods. Preliminary phytochemical screening of MECP was done by standard procedure. Synthesis of silver nanoparticles from MECP was done. In-vitro anti-oxidant activity of the MECP & MECPAgNPs were studied by DPPH assay, H2O2 scavenging activity, Phosphomolybdenum Method, FRAP and reducing power assay. The total Phenolic content, Flavonoid content & Vitamin C were estimated by using Gallic acid, Quercetin and standard Ascorbic acid calibration curve respectively. Preliminary phytochemical screening showed the presence of carbohydrates, triterpenoids, proteins, alkaloids, tannins, saponins, flavonoids, sterols and volatile oil. In-vitro antioxidant methods were resulted, the extract and the nanoparticles showed a dose dependent reducing ability. The nanoparticles at the same concentration offered much better activity than the extract alone. Phenolic content, Flavonoid content and Vitamin C amount of the MECPAgNPs was higher than MECP. These determination and quantification gives the information about the amount of secondary metabolites present in the MECPAgNPs was higher than the MECP which is responsible for the therapeutic or pharmacological activity of the plant. The MECPAgNPs showed very potent anti oxidant activity as compared to MECP.
Keywords:
Costus pictus, In-vitro anti-oxidant, 1, 1-diphenyl-2-picryl hydrazyl (DPPH), Total Phenolic Content, Flavonoid contentDOI
https://doi.org/10.25004/IJPSDR.2014.060411References
1. Ames BN, Shigenega MK, Hagen TM. Oxidants and the degenerative diseases of aging. Proc Nati Acad Sci. 1993; 90: 7915-7922.
2. Shenoy R, Shirwaikar A. Anti-inflammatory and free radical scavenging studies of Hyptis suaveolens (Labiatae). Indian drugs 2002; 39: 574-577.
3. Evans P, Halliwall B. Free radicals and hearing. Ann N Y Acad Sci. 1999; 884: 19-40.
4. Devasagayam TPA, Kesavan PC. Radio protective and antioxidant action of Caffeine mechanistic considerations. Indian J Exptl Biol. 2003; 41: 267-269.
5. Peterhans E. Oxidants and antioxidants in viral diseases; disease mechanisms and metabolic regulation. J. Nutr. 1997; 127 (Suppl 5): 962-965.
6. Sies H, Stahl W. Withania somnifera, Vitamin E and C, β-carotene and other Carotenoids as antioxidants. Am J Clin Nutr. 1995; 62: 1315-1321.
7. Parker RS. Dietary and biochemical aspects of Vitamin E. Adv Food Nutr Res. 1980; 33: 157-232.
8. Nur Alam Md, Bristi NJ, Rafiquzzaman Md. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal 2013; 21(2): 143-152.
9. Jose B, Reddy LJ. Analysis of the essential oils of the stems, leaves and rhizomes of the medicinal plant Costus pictus from Southern India. Int J Pharmacy Pharm Sci. 2010; 2 (Suppl 2): 100-101.
10. Aruna A, Nandhini SR, Karthikeyan V, Bose P, Vijayalakshmi K, Jegadeesh S. Insulin plant (Costus pictus) leaves: pharmacognostical standardization and phytochemical evaluation. American Journal of Pharmacy & Health Research 2014; 2(8): 106-119.
11. Modak M, Dixit P, Londhe J, Ghaskadbi S, Paul T, Devasagayam A. Indian Herbs and Herbal Drugs used for the treatment of Diabetes. J Clin Biochem Nutr. 2007 May; 40(3): 163-173.
12. Jayasri MA, Gunasekaran S, Radha A, Mathew TL. Anti-diabetic effect of Costus pictus leaves in normal and streptozotocin-induced diabetic rats. Int J Diabetes and Metabolism 2008; 16: 117-122.
13. Gireesh G, Thomas SK, Joseph B, Paulose CS. Antihyperglycemic and insulin secretory activity of Costus pictus leaf extract in streptozotocin induced diabetic rats and in in vitro pancreatic islet culture. J Ethno Pharmacol. 2009; 123: 470-474.
14. Jothivel N, Ponnusamy SP, Appachi M, Singaravel S, Rasilingam D, Deivasigamani K et al. Anti-diabetic activity of Methanol leaf extract of Costus pictus D. Don in alloxan-induced diabetic rats. J Health Sci. 2007; 53: 655-663.
15. Pareek A, Suthar M, Godavarthi A, Goyal M, Bansal V. Negative regulation of glucose uptake by Costus pictus in L6 myotube cell line. J Pharm Negative 2010; 1: 24-26.
16. Al-Romaiyan A, Jayasri MA, Mathew TL, Huang GC, Amiel S, Jones PM et al. Costus pictus extracts stimulate Insulin secretion from mouse and human islets of Langerhans in vitro. Cell Physiol Biochem. 2010; 26: 1051-1058.
17. Isaac ST, Alphonse JK. Comparative study of hypoglycemic activity of Costus pictus and Costus igneus in streptozotocin induced diabetic rat. J Pharm Res. 2011; 4: 3628-3629.
18. Suganya S, Narmadha R, Gopalakrishnan VK, Devaki K. Hypoglycemic effect of Costus pictus D. Don on alloxan induced type 2 diabetes mellitus in albino rats. Asian Pac J Trop Disease 2012; 2: 117-123.
19. Mani P, Kumar AR, Bastin TM, Jenifer S, Arumugam M. Comparative evaluation of extracts of C. igneus (or C. pictus) for hypoglycemic and hypolipidemic activity in alloxan diabetic rats. Int J Pharm Tech. 2010; 2: 183-195.
20. Reddy LJ, Jose B. Evaluation of antibacterial activity of the essential oil of Costus pictus D. Don from South India. International Journal of Current Pharmaceutical Research 2010; 2(3): 68-70.
21. Majumdar M, Parihar PS. Antibacterial, antioxidant and antiglycation potential of Costus pictus from Southern region, India. Asian J Plant Sci Res. 2012; 2: 95-101.
22. Nadumane VK, Rajashekar S, Narayana P, Adinarayana S, Vijayan S, Prakash S et al. Evaluation of the anticancer potential of Costus pictus on fibrosarcoma (HT-1080) cell line. J Nat Pharm. 2011; 2: 72-76.
23. Caceres A, Grion LM, Martinez AM. Diuretic activity of plants used for the treatment of urinary ailments in Guatemala. J Ethnopharmacol.1987; 19: 233-245.
24. Comargo MEM, Najera CR, Torres RS, Alderate MEC. Evaluation of the diuretic effect of the aqueous extract of Costus pictus D. Don in rat. Poc West Pharmacol Soc. 2006; 49: 72-74.
25. Jayasri MA, Mathew L, Radha A. A report on the antioxidant activity of leaves and rhizomes of Costus pictus D. Don. Int J Integr Biol. 2009; 5 (1): 20-26.
26. Sethumathi PP, Nandhakumar J, Sengottuvelu S, Duraisamy R, Karthikeyan D, Ravikumar VR et al. Anti diabetic and antioxidant activity of Methanolic leaf extracts of Costus pictus D. Don in alloxan induced diabetic rats. Pharmacology online 2009; 1: 1200-1213.
27. Khandelwal KR. Practical Pharmacognostic Techniques and Experiments. 19th ed. Nirali Prakashan, Pune; 2008.
28. Harbone JB. Phytochemical Analysis - A guide to modern techniques of plant analysis. 1st ed. Chapman & Hall, New York: 1980.
29. Aruna A, Nandhini SR, Karthikeyan V, Bose P. Synthesis and Characterization of Silver Nanoparticles of Insulin Plant (Costus pictus D. Don) Leaves. Asian Journal of Biomedical and Pharmaceutical Sciences 2014; 4(34): 1-6.
30. Panda S, Tripathy JK, Panda JR. A comparative study of antioxidant properties of 2-[substituted arylideamino]-1, 3, 4 thiadiazino [6,5B] indoles and their inclusion complexes with β-Cyclodextrin. International Journal of Pharmaceutical Sciences and Drug Research 2012; 4(3): 191-194.
31. Brand Williams W, Cuvelier ME, Berset C. Use of Free Radical method to evaluate Antioxidant activity. Lebensm Wiss Technol, 1995; 28: 25-30.
32. Sahgal G, Ramanathan S, Sashidharan S, Mordi MN, Ismail S, Mansoor SM. In vitro antioxidant and xanthine oxidase inhibitory activities of Methanolic Swietenia mahogoni seed extracts. Molecules 2009; 14: 4476-4485.
33. Aruna A, Vijayalakshmi K, Karthikeyan V. In-vitro antioxidant screening of Citrullus lanatus leaves. International Journal of Pharmacy and Pharmaceutical Analysis 2014; 1(1): 9-26.
34. Pal DK, Dutta S. Evaluation of antioxidant activity of the roots of Cyperus rotundus L. Indian J Pharm Sci. 2006; 68: 256-258.
35. Pal DK, Kumar M, Chakraborty P, Kumar S. Evaluation of antioxidant activity of aerial parts of Cynodon dactylon. Asian J Chem. 2008; 20: 2479-2481.
36. Yaya AJG, Feumba RD, Emmanuel T, Tchinda AT, Fredérich M, Oben J et al. Antioxidant Activity of Compounds Isolated from the Root Woods of Erythrina droogmansiana. International Journal of Pharmaceutical Sciences and Drug Research 2014; 6(2): 160-163.
37. Sannigrahi S, Mazumder UK, Pal DK, Parida S. In vitro antioxidant activity of Methanol extract of Clerodendrum infortunatum. Orient Pharm Exp Med. 2009; 9: 128-134.
38. Jayanthi P, Lalitha P, Reducing Power of the of Eichhornia crassipes (Mart.) Solms, Int J Pharm Pharm Sci. 2011; 3(3): 126-128.
39. Pal DK, Nimse SB. Screening of antioxidant activity of Hydrilla verticillata plant. Asian J Chem. 2006; 18: 3004-3008.
40. Patel A, Patel A, Patel NM. Estimation of Flavonoid, polyphenolic content and in-vitro antioxidant capacity of leaves of Tephrosia purpurea Linn. (Leguminosae). Int J Pharm. Sci Res. 2010; 1(1): 66-77.
41. Jain P, Jain S, Pareek A, Sharma S. A comprehensive study on the natural plant phenols: perception to current scenario. Bulletin of Pharmaceutical Research 2013; 3(2): 90-106.
42. Siddque MA, Mujeeb M, Najim AK, Akram M. Evaluation of antioxidant activity, quantitative estimation of phenols and flavonoids in different parts of Aegle marmelos. African J plant Sci. 2010; 4(1): 1-5.
43. Mabry TJ, Markham KR, Thomas MB. The systematic identification of flavonoids. Springer Verlay, New York, USA: 1970.
44. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total Flavonoid content in Propolis by two complementary colorimetric methods. J Food Drug Analysis 2002; 10(3): 178-182.
45. Aruna A, Nandhini SR, Karthikeyan V, Bose P, Vijayalakshmi K. Comparative anti-Diabetic effect of Methanolic extract of Insulin Plant (Costus pictus) leaves and its silver nanoparticle. Indo American Journal of Pharmaceutical Research 2014; 4(7): 3217-3230.
46. Li Y, Schell horn HE. New developments and novel therapeutic perspectives for Vitamin C. J Nutr. 2007; 137: 71-84.
47. Luqman S, Rizvi SI. Protection of lipid peroxidation and carbonyl formation in proteins by capsaicin in human erythrocytes subjected to oxidative stress. Phytother Res. 2006; 20: 303-306.
48. Pandey KB, Mishra N, Rizvi SI. Protective role of Myricetin on markers of oxidative stress in human erythrocytes subjected to oxidative stress. Nat Prod Commun. 2009; 4: 221-226.
49. Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity 2009; 2(5): 270-278.
50. Rizvi SI, Mishra M. Anti-oxidant effect of Quercetin on type 2 diabetic erythrocytes. J Food Biochem. 2009; 33:404-415.
51. Groot H, Rauen U. Tissue injury by reactive oxygen species and the protective effects of Flavonoids. Fundam Clin Pharmacol. 1998; 12: 249-255.
52. Rekha C, Poornima G, Manasa M, Abhipsa V, Pavithra devi J, Vijay Kumar HT, Prashith Kekuda TR. Ascorbic acid, total Phenol content and Antioxidant activity of fresh juices of four ripe and unripe Citrus fruits. Chem Sci Trans. 2012; 1(2): 303-310.
2. Shenoy R, Shirwaikar A. Anti-inflammatory and free radical scavenging studies of Hyptis suaveolens (Labiatae). Indian drugs 2002; 39: 574-577.
3. Evans P, Halliwall B. Free radicals and hearing. Ann N Y Acad Sci. 1999; 884: 19-40.
4. Devasagayam TPA, Kesavan PC. Radio protective and antioxidant action of Caffeine mechanistic considerations. Indian J Exptl Biol. 2003; 41: 267-269.
5. Peterhans E. Oxidants and antioxidants in viral diseases; disease mechanisms and metabolic regulation. J. Nutr. 1997; 127 (Suppl 5): 962-965.
6. Sies H, Stahl W. Withania somnifera, Vitamin E and C, β-carotene and other Carotenoids as antioxidants. Am J Clin Nutr. 1995; 62: 1315-1321.
7. Parker RS. Dietary and biochemical aspects of Vitamin E. Adv Food Nutr Res. 1980; 33: 157-232.
8. Nur Alam Md, Bristi NJ, Rafiquzzaman Md. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal 2013; 21(2): 143-152.
9. Jose B, Reddy LJ. Analysis of the essential oils of the stems, leaves and rhizomes of the medicinal plant Costus pictus from Southern India. Int J Pharmacy Pharm Sci. 2010; 2 (Suppl 2): 100-101.
10. Aruna A, Nandhini SR, Karthikeyan V, Bose P, Vijayalakshmi K, Jegadeesh S. Insulin plant (Costus pictus) leaves: pharmacognostical standardization and phytochemical evaluation. American Journal of Pharmacy & Health Research 2014; 2(8): 106-119.
11. Modak M, Dixit P, Londhe J, Ghaskadbi S, Paul T, Devasagayam A. Indian Herbs and Herbal Drugs used for the treatment of Diabetes. J Clin Biochem Nutr. 2007 May; 40(3): 163-173.
12. Jayasri MA, Gunasekaran S, Radha A, Mathew TL. Anti-diabetic effect of Costus pictus leaves in normal and streptozotocin-induced diabetic rats. Int J Diabetes and Metabolism 2008; 16: 117-122.
13. Gireesh G, Thomas SK, Joseph B, Paulose CS. Antihyperglycemic and insulin secretory activity of Costus pictus leaf extract in streptozotocin induced diabetic rats and in in vitro pancreatic islet culture. J Ethno Pharmacol. 2009; 123: 470-474.
14. Jothivel N, Ponnusamy SP, Appachi M, Singaravel S, Rasilingam D, Deivasigamani K et al. Anti-diabetic activity of Methanol leaf extract of Costus pictus D. Don in alloxan-induced diabetic rats. J Health Sci. 2007; 53: 655-663.
15. Pareek A, Suthar M, Godavarthi A, Goyal M, Bansal V. Negative regulation of glucose uptake by Costus pictus in L6 myotube cell line. J Pharm Negative 2010; 1: 24-26.
16. Al-Romaiyan A, Jayasri MA, Mathew TL, Huang GC, Amiel S, Jones PM et al. Costus pictus extracts stimulate Insulin secretion from mouse and human islets of Langerhans in vitro. Cell Physiol Biochem. 2010; 26: 1051-1058.
17. Isaac ST, Alphonse JK. Comparative study of hypoglycemic activity of Costus pictus and Costus igneus in streptozotocin induced diabetic rat. J Pharm Res. 2011; 4: 3628-3629.
18. Suganya S, Narmadha R, Gopalakrishnan VK, Devaki K. Hypoglycemic effect of Costus pictus D. Don on alloxan induced type 2 diabetes mellitus in albino rats. Asian Pac J Trop Disease 2012; 2: 117-123.
19. Mani P, Kumar AR, Bastin TM, Jenifer S, Arumugam M. Comparative evaluation of extracts of C. igneus (or C. pictus) for hypoglycemic and hypolipidemic activity in alloxan diabetic rats. Int J Pharm Tech. 2010; 2: 183-195.
20. Reddy LJ, Jose B. Evaluation of antibacterial activity of the essential oil of Costus pictus D. Don from South India. International Journal of Current Pharmaceutical Research 2010; 2(3): 68-70.
21. Majumdar M, Parihar PS. Antibacterial, antioxidant and antiglycation potential of Costus pictus from Southern region, India. Asian J Plant Sci Res. 2012; 2: 95-101.
22. Nadumane VK, Rajashekar S, Narayana P, Adinarayana S, Vijayan S, Prakash S et al. Evaluation of the anticancer potential of Costus pictus on fibrosarcoma (HT-1080) cell line. J Nat Pharm. 2011; 2: 72-76.
23. Caceres A, Grion LM, Martinez AM. Diuretic activity of plants used for the treatment of urinary ailments in Guatemala. J Ethnopharmacol.1987; 19: 233-245.
24. Comargo MEM, Najera CR, Torres RS, Alderate MEC. Evaluation of the diuretic effect of the aqueous extract of Costus pictus D. Don in rat. Poc West Pharmacol Soc. 2006; 49: 72-74.
25. Jayasri MA, Mathew L, Radha A. A report on the antioxidant activity of leaves and rhizomes of Costus pictus D. Don. Int J Integr Biol. 2009; 5 (1): 20-26.
26. Sethumathi PP, Nandhakumar J, Sengottuvelu S, Duraisamy R, Karthikeyan D, Ravikumar VR et al. Anti diabetic and antioxidant activity of Methanolic leaf extracts of Costus pictus D. Don in alloxan induced diabetic rats. Pharmacology online 2009; 1: 1200-1213.
27. Khandelwal KR. Practical Pharmacognostic Techniques and Experiments. 19th ed. Nirali Prakashan, Pune; 2008.
28. Harbone JB. Phytochemical Analysis - A guide to modern techniques of plant analysis. 1st ed. Chapman & Hall, New York: 1980.
29. Aruna A, Nandhini SR, Karthikeyan V, Bose P. Synthesis and Characterization of Silver Nanoparticles of Insulin Plant (Costus pictus D. Don) Leaves. Asian Journal of Biomedical and Pharmaceutical Sciences 2014; 4(34): 1-6.
30. Panda S, Tripathy JK, Panda JR. A comparative study of antioxidant properties of 2-[substituted arylideamino]-1, 3, 4 thiadiazino [6,5B] indoles and their inclusion complexes with β-Cyclodextrin. International Journal of Pharmaceutical Sciences and Drug Research 2012; 4(3): 191-194.
31. Brand Williams W, Cuvelier ME, Berset C. Use of Free Radical method to evaluate Antioxidant activity. Lebensm Wiss Technol, 1995; 28: 25-30.
32. Sahgal G, Ramanathan S, Sashidharan S, Mordi MN, Ismail S, Mansoor SM. In vitro antioxidant and xanthine oxidase inhibitory activities of Methanolic Swietenia mahogoni seed extracts. Molecules 2009; 14: 4476-4485.
33. Aruna A, Vijayalakshmi K, Karthikeyan V. In-vitro antioxidant screening of Citrullus lanatus leaves. International Journal of Pharmacy and Pharmaceutical Analysis 2014; 1(1): 9-26.
34. Pal DK, Dutta S. Evaluation of antioxidant activity of the roots of Cyperus rotundus L. Indian J Pharm Sci. 2006; 68: 256-258.
35. Pal DK, Kumar M, Chakraborty P, Kumar S. Evaluation of antioxidant activity of aerial parts of Cynodon dactylon. Asian J Chem. 2008; 20: 2479-2481.
36. Yaya AJG, Feumba RD, Emmanuel T, Tchinda AT, Fredérich M, Oben J et al. Antioxidant Activity of Compounds Isolated from the Root Woods of Erythrina droogmansiana. International Journal of Pharmaceutical Sciences and Drug Research 2014; 6(2): 160-163.
37. Sannigrahi S, Mazumder UK, Pal DK, Parida S. In vitro antioxidant activity of Methanol extract of Clerodendrum infortunatum. Orient Pharm Exp Med. 2009; 9: 128-134.
38. Jayanthi P, Lalitha P, Reducing Power of the of Eichhornia crassipes (Mart.) Solms, Int J Pharm Pharm Sci. 2011; 3(3): 126-128.
39. Pal DK, Nimse SB. Screening of antioxidant activity of Hydrilla verticillata plant. Asian J Chem. 2006; 18: 3004-3008.
40. Patel A, Patel A, Patel NM. Estimation of Flavonoid, polyphenolic content and in-vitro antioxidant capacity of leaves of Tephrosia purpurea Linn. (Leguminosae). Int J Pharm. Sci Res. 2010; 1(1): 66-77.
41. Jain P, Jain S, Pareek A, Sharma S. A comprehensive study on the natural plant phenols: perception to current scenario. Bulletin of Pharmaceutical Research 2013; 3(2): 90-106.
42. Siddque MA, Mujeeb M, Najim AK, Akram M. Evaluation of antioxidant activity, quantitative estimation of phenols and flavonoids in different parts of Aegle marmelos. African J plant Sci. 2010; 4(1): 1-5.
43. Mabry TJ, Markham KR, Thomas MB. The systematic identification of flavonoids. Springer Verlay, New York, USA: 1970.
44. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total Flavonoid content in Propolis by two complementary colorimetric methods. J Food Drug Analysis 2002; 10(3): 178-182.
45. Aruna A, Nandhini SR, Karthikeyan V, Bose P, Vijayalakshmi K. Comparative anti-Diabetic effect of Methanolic extract of Insulin Plant (Costus pictus) leaves and its silver nanoparticle. Indo American Journal of Pharmaceutical Research 2014; 4(7): 3217-3230.
46. Li Y, Schell horn HE. New developments and novel therapeutic perspectives for Vitamin C. J Nutr. 2007; 137: 71-84.
47. Luqman S, Rizvi SI. Protection of lipid peroxidation and carbonyl formation in proteins by capsaicin in human erythrocytes subjected to oxidative stress. Phytother Res. 2006; 20: 303-306.
48. Pandey KB, Mishra N, Rizvi SI. Protective role of Myricetin on markers of oxidative stress in human erythrocytes subjected to oxidative stress. Nat Prod Commun. 2009; 4: 221-226.
49. Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity 2009; 2(5): 270-278.
50. Rizvi SI, Mishra M. Anti-oxidant effect of Quercetin on type 2 diabetic erythrocytes. J Food Biochem. 2009; 33:404-415.
51. Groot H, Rauen U. Tissue injury by reactive oxygen species and the protective effects of Flavonoids. Fundam Clin Pharmacol. 1998; 12: 249-255.
52. Rekha C, Poornima G, Manasa M, Abhipsa V, Pavithra devi J, Vijay Kumar HT, Prashith Kekuda TR. Ascorbic acid, total Phenol content and Antioxidant activity of fresh juices of four ripe and unripe Citrus fruits. Chem Sci Trans. 2012; 1(2): 303-310.
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01-10-2014
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“COMPARATIVE IN-VITRO ANTIOXIDANT SCREENING OF METHANOLIC EXTRACT OF COSTUS PICTUS & ITS SILVER NANOPARTICLES”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 4, Oct. 2014, pp. 334-40, https://doi.org/10.25004/IJPSDR.2014.060411.
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How to Cite
“COMPARATIVE IN-VITRO ANTIOXIDANT SCREENING OF METHANOLIC EXTRACT OF COSTUS PICTUS & ITS SILVER NANOPARTICLES”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 4, Oct. 2014, pp. 334-40, https://doi.org/10.25004/IJPSDR.2014.060411.
