DIFFERENTIAL PULSE POLAROGRAPHIC DETERMINATION OF NIFEDIPINE IN PHARMACEUTICAL FORMULATIONS
Abstract
A simple and rapid differential pulse polarographic method has been developed for the trace determination of Nifedipine. A well-defined single peak with Ep value of -0.51 V is obtained in 0.1M acetate buffer (pH 5.0). The linearity is valid up to 5×10-5 M (r =0.9995) with minimum detection limit of 3.5×10-8 M. Precision of the method developed is implied from the values of relative mean deviation, standard deviation and coefficient of variation, which are 2.05%, 1.1 and 3.2% respectively. Marketed formulations of Nifedipine have been analyzed by calibration and standard addition methods. Recovery experiments have been found to be quantitative and analysis to determine the mass per tablet is obtained within ± 0.2% of the expected value. The studies have shown that the method is simple, reproducible and accurate and can be used in the analysis of marketed formulations.
Keywords:
Differential pulse polarography, Nifedipine, pharmaceutical.DOI
https://doi.org/10.25004/IJPSDR.2011.030318References
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10. Senturk Z, Ozkan S A, Ozkan Y. Electroanalytical study of nifedipine using activated glassy carbon electrode. J Pharm Biomed Anal. 1998; 16: 801-807.
11. Shapovalov VA. Determination of Nifedipine by Differential Pulse Adsorptive Stripping Polarography. J Anal Chem. 2002; 57: 157-158.
12. Alvarez-Lueji A, Nunez-Vergara LJ, Squella JA. Voltammetric behavior of 1, 4-dihydropyridine calcium antagonists. Electroanalysis. 1994; 6: 259-264.
13. Squellaa JA, Barnafib E, Pernac S, Nuñez-Vergarac LJ. Nifedipine: differential pulse polarography and photo-decomposition. Talanta. 1989; 36: 363-366.
14. Galus Z. Fundamentals of electrochemical analysis (1st edn). Ellis Horwood England, 1976.
2. Asthana S, Kaur V, Chawla P, Saraf SA. Rapid and Sensitive HPLC-UV method for Simultaneous Estimation of Nifedipine, Nateglinide and Lovastatin: Quantitative Application to Polypill Based Synthetic Ternary Mixture. Int J PharmTech Res. 2010; 2: 682-688.
3. Bruno L, John SK, New spectrophotometric methods for estimation of nifedipine. Ind J Pharma Sci. 1988; 50: 109-112.
4. Tanner R, Romagnoli A, Kramer WG. Simplified method for determination of plasma nifedipine by gas chromatography. J Anal Toxicol; 1986; 10: 250-251.
5. Al-Ghannam SM, Al-Olyan AM. Spectrofluorometric determination of nicardipine, nifedipine and isradipine in pharmaceutical preparations and biological fluids. Central European J Chem. 2008; 6: 222-228.
6. Abou-Auda HS, Najjar TA, Al-Khamis KI, Al-Hadiya BM, Ghilzai NM, Al-Fawzan NF. Liquid chromatographic assay of nifedipine in human plasma and its application to pharmacokinetic studies. J Pharm Biomed Anal. 2000; 22: 241-249.
7. Horvath V, Hrabeczy-Pall A, Niegreisz Z, Kocsi E, Horvai G, Godorhazy L, Tolokan A, Kleborich I, Balogh-Nemes K. Sensitive high-performance liquid chromatographic determination of nifedipine in dog plasma using an automated sample preparation system with laboratory robot. J Chromatogr B: Biomed. Sci Appl. 1996; 686: 211-219.
8. Nakamura I, Takahashi M, Izumi H. Sensitive high-performance liquid chromatographic determination of nifedipine in cat plasma following improved sample treatment. J Chromatogr B: Biomed Sci Appl, 1999; 729: 265-270.
9. Reddy TM, Reddy SJ. Differential Pulse Adsorptive Stripping Voltammetric Determination of Nifedipine and Nimodipine in Pharmaceutical Formulations, Urine, and Serum Samples by Using a Clay-Modified Carbon-Paste Electrode. Anal Lett. 2004; 37: 2079-2098.
10. Senturk Z, Ozkan S A, Ozkan Y. Electroanalytical study of nifedipine using activated glassy carbon electrode. J Pharm Biomed Anal. 1998; 16: 801-807.
11. Shapovalov VA. Determination of Nifedipine by Differential Pulse Adsorptive Stripping Polarography. J Anal Chem. 2002; 57: 157-158.
12. Alvarez-Lueji A, Nunez-Vergara LJ, Squella JA. Voltammetric behavior of 1, 4-dihydropyridine calcium antagonists. Electroanalysis. 1994; 6: 259-264.
13. Squellaa JA, Barnafib E, Pernac S, Nuñez-Vergarac LJ. Nifedipine: differential pulse polarography and photo-decomposition. Talanta. 1989; 36: 363-366.
14. Galus Z. Fundamentals of electrochemical analysis (1st edn). Ellis Horwood England, 1976.
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01-07-2011
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“DIFFERENTIAL PULSE POLAROGRAPHIC DETERMINATION OF NIFEDIPINE IN PHARMACEUTICAL FORMULATIONS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 3, July 2011, pp. 253-5, https://doi.org/10.25004/IJPSDR.2011.030318.
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How to Cite
“DIFFERENTIAL PULSE POLAROGRAPHIC DETERMINATION OF NIFEDIPINE IN PHARMACEUTICAL FORMULATIONS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 3, July 2011, pp. 253-5, https://doi.org/10.25004/IJPSDR.2011.030318.
