EFFECT OF TEMPERATURE AND LIGHT ON PHYTOCHEMICAL PROFILING AND ANTIMICROBIAL ACTIVITY OF ANDROGRAPHIS PANICULATA
Abstract
Andrographis paniculata (Acanthaceae) is an annual herb. It is found in Sri Lanka, and throughout the plains of India especially Tamilnadu, Maharashtra, Karnataka, and Orissa. Various medicinal properties like cholevetic, antidiarrheal, immunostimulant and anti-inflammatory have been attributed to this plant in the traditional system of Indian medicine. Further reported activities are hepatoprotective, antimalarial, anticancer, antihypertensive, antipyretic, antithrombotic and antidote for snake bites. The present study aimed to evaluate the anti-microbial activity for the isopropanol extract of A. paniculata against different bacterial strains such as E. coli, K. pneumoniae, P. vulgaris, S. pneumoniae, P. aeruginosa, S. aureus by determining inhibitory concentration and zone of inhibition. Minimum inhibitory concentration and zone of inhibition values and the high bioactive metabolites production was observed in different extracts of A. paniculata under different physical stress (Light and temperatures). The results revealed that, the isopropanol extract of A. paniculata is potent for inhibiting bacterial growth and various secondary metabolites production in dark condition at 37°C than other tested parameters.
Keywords:
Antimicrobial activity, Phytochemical analysis, Minimum Inhibitory Concentration, Andrographis paniculataDOI
https://doi.org/10.25004/IJPSDR.2015.070609References
2. Williams R. Antimicrobial resistance a global threat. Esse. Drug. Moni. 2000; 13: 28-29.
3. Pretorius JC, Magama S, Zietsman PC. Growth inhibition of plant pathogenic bacteria and fungi by extracts from selected South African plant species. South African J. Bot. 2003; 20: 188-192.
4. Leelavasamee A. Undetectable antibacterial activity of A. paniculata (Burn) wall ex Nees. J. med. Assoc. of Thailand. 1990; 73:299-304.
5. Balandrin MF, Kjocke AJ, Wurtele E. Natural plant chemicals: sources of industrial and mechanical materials. Sci. 1985; 228: 1154-1160.
6. Li W, Xu X, Zhang H. Secondary metabolites from A. paniculata. Chem. Pharm. Bull. (Tokyo) 2007; 55:455-458.
7. Krishnaraju AV, Rao TVN, Sundararaju D. Assessment of bioactivity of Indian medicinal plants using Brine shrimp (Artemia salina) lethality assay. Int. J. Appl. Sci. Eng. 2005; 2: 125-134.
8. Cacer DD, Hancke JL, Burgos RLA, Wickman JK. Prevention of common cold with A. paniculata dried extract. A pilot double blind trial. Phytomed.1997; 4: 101-104.
9. Upadhyaya MK, Furness NH. Influence of light intensity and water stress on leaf surface characteristics of Cynoglossum offıcinale, Centaurea spp. and Tragopogon spp., Can. J. Bot. 1994; 72:1379–1386.
10. Mishra S, Tiwari SK, Kakker A, Pandey AK. Chemoprofiling of A. paniculata (Kelmegh) for its andrographolide content in Madhya Pradesh, India. Int. J. Pharm. Biol. Sci, 2010; 1 (2): 1-5.
11. Trivedi NP, Rawal UM. Hepatoprotetive and antioxidant property of A. paniculata in BHC-induced liver damage in mice. Indian J. Exp.Biol. 2001; 39:41-46.
12. Zhao J, Davis LC, Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv. 2005; 23: 283-333.
13. Bauer AW, Kirby WMM, Sherris JC. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin Pathol. 1966; 45: 493-496.
14. Gupta S. Antidiarrheal activity of diterpenes of A. paniculata (kalamegh) against E. coli enterotoxin in in vivo models. Int. J. Crude. Drug. Res.1990; 28: 273-283.
15. Bourgaud F, Gravot A, Milesi S, Gontier E. Production of plant secondary metabolites: a historical perspective. Plant Sci. 2001; 161: 839–851.
16. Odabas MS, Uzun S, Gulumser A. The Quantitative Effects of Temperature and Light on Growth, Development and Yield of Faba Bean (Vicia faba L.): I. Growth. Int. J. Agric. Res. 2007; 2(9):765-775.
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