EVALUATION OF IN VITRO ANTIVIRAL ACTIVITY OF HYPERICUM MYSORENSE F. HEYNE AGAINST HSV-2
Abstract
The medicinal plants have played a vital role in drug discovery. Many plants are used traditionally for the treatment of different types of viral disorders and other infectious diseases. Herpes simplex virus (HSV) is a ubiquitous organism that causes infections in human populations throughout the world. It causes a variety of diseases ranging in severity from mild to life-threatening. The present study was to evaluate antiviral activity of Hypericum mysorense F. Heyne ethanolic leaf extract against herpes simplex virus type 2 (HSV-2) using HEp-2 cell line. The toxic free concentrations of ethanolic extract were determined using MTT assay, followed by anti HSV-2 activity and a positive control was maintained. 50µg/ml of ethanol extract was observed to be maximum non toxic free concentration against HEp-2 cell line and also exhibited significant inhibitory activity against HSV-2.
Keywords:
Hypericum mysorense, HEp-2 cell line, MTT assay, Antiviral.DOI
https://doi.org/10.25004/IJPSDR.2017.090106References
Vanden Berghe DA, Vlietinck AJ, Vanhoof L. Plant products as potential antiviral agents. Bull Inst Pausteur. 1986; 84:101-147.
Mandal M. Progress and Prospects in Therapeutics against HIV Infection. Int. J. Pharm. Sci. Drug Res. 2015; 7(6): 432-443.
Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. Ethnopharmacological approaches to wound healing exploring medicinal plants of India. Journal Ethnopharmacol. 2007:114(2): 103-113.
Vijayan P, Raghu C, Ashok G, Dhanaraj SA, Suresh B. Antiviral activity of medicinal plants of Nilgiris. Indian J Med Res. 2004; 120: 24-29.
Schiffer JT, Corey L. New concepts in understanding genital herpes. Curr Infect Dis Rep. 2009; 11(6): 457–64.
Field AK, Biron KK. “The end of innocence” revisited: Resistance of herpeviruses to antiviral drugs. Clin microbial rev.1944; 7:1-13.
Good NE, winget Gd, winder W, connoly TN, Izawa S, Sing RMM. Hydrogen iron buffer for virological research. Bioche. 1966; 5:467-477.
Mosmann. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays; Journal of Immunological Methods. 1983; 65: 55-63 .
Reed LJ & Muench H. A simple method of estimating fifty percent endpoints. Am J Hygiene 1938; 27:493-497.
The wealth of India, New Delhi: Council of Scientific and Industrial Research, 1995, pp. 5-6.
Benencia F, Courreges C. Antiviral activity of Sandal wood oil against herpes simplex viruses-1 and -2. Phytomedicine. 1999; 6:119-123.
Lavie G, Mazur Y, Lavie D, Meruelo D. The chemical and biological properties of hypericin a compound with a broad spectrum of biological activities. Med. Res. Rev. 1995; 15(2): 111-119.
Cos P, Hermans N, De Bruyne T, Apers S, Sindambiwe JB, Vanden Berghem D, Pieters L, Vlietinck AJ. Further evaluation of Rwandan medicinal plant extracts for their antimicrobial and antiviral activities. J. Ethnopharmacol. 2002; 79(2): 155-163.
Published
Abstract Display: 1180 
PDF Downloads: 619 
