ACUTE TOXICITY, IN-VITRO UROLITHIATIC & DIURETIC EVALUATION OF METHANOLIC EXTRACT OF HYGROPHILA SALICIFOLIA, WHOLE HERB IN RAT
Abstract
Hygrophila salicifolia (Family: Acanthaceae) is an erect herb, nearly glabrous, mainly whole herb used as raw material in medicine. The aim of present investigation was to evaluate acute toxicity, in vitro urolithiatic and diuretic activity of methanolic extract of Hygrophila salicifolia, whole herb. Methanolic extract of Hygrophila salicifolia was administered to experimental rats orally at 2000 mg/kg p.o. The acute toxicity of the extract was evaluated as per OECD guideline 420. The LD50 of the extract was found to be between 2000-5000 mg/kg p.o. Urolithiatic activity was determined for all extracts using Conductometric titration and crystal growth inhibition. Methanolic extract of Hygrophila salicifolia was administered to experimental rats orally at doses 300 & 500 mg/kg p.o. Furosemide (10 mg/kg) was used as positive control. The diuretic potential of the extracts was evaluated by measuring urine volume, Diuretic index, Lipchitz value, Saliuretic index, Na+/K+ ratio and excretion of sodium-potassium content. The present study provided quantitative and qualitative basis for explaining non toxicity, urolithiatic and diuretic potential of methanolic extract of Hygrophila salicifolia.
Keywords:
Hygrophila salicifolia, Acute toxicity, Urolithiatic, Diuretic activity, Methanolic extractDOI
https://doi.org/10.25004/IJPSDR.2016.080208References
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