ANTI-UROLITHIATIC POTENTIAL OF METHANOLIC EXTRACT OF HYGROPHILA SALICIFOLIA ON ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATSq
Abstract
The anti-urolithiatic action of Hygrophila salicifolia (Acanthaceae) was determined by ethylene glycol induced lithiasis. Ethylene glycolated water (0.75% v/v) was given to experimental animals for 28 days to produce stone in kidney. Methanolic extracts of Hygrophila salicifolia were given from the 1st day as inhibitory agent. Urinary parameters such as calcium, oxalate and inorganic phosphate level were raised by ethylene glycol where as test extract lowered down the raised level of these ions in urine. Methanolic extract of Hygrophila salicifolia also increased urinary magnesium concentration while raised concentration of serum creatinine was found to be cut down during the experiment. Methanolic extract of test drug was found impressively better from histological study. These results obtained from the study confirmed the anti-urolithiatic potential of Hygrophila salicifolia.
Keywords:
Hygrophila salicifolia, Ethylene glycol, Anti-urolithiatic actionDOI
https://doi.org/10.25004/IJPSDR.2016.080602References
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13. Ravindra VK, Navneet BG, Alagawadi KR, Rudraprabhu VS. Ethylene glycol induced urolithiasis in effect of Moringa oleifera Lam. Root‐wood on rats. Journal of Ethnopharamacology2006; 105(3): 306‐311.
14. Mustafa MA, Medeiros DM. Proximate composition, mineral content and fatty acids of cat fish for different seasons and cooking methods. J Food Sci. 1985; 50: 585‐588.
15. Hodgkinson A, Williams A. An improved colorimetric procedure for urine oxalate. Clin Chim Acta. 1972; 36(2):127‐132.
16. Fiske CH, Subbarow Y. The colorimetric determination of phosphorus. J Biol Chem. 1925; 66:375‐381.
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement using Folia‐ciocalteau reagent. J Biol Chem. 1951; 193(7):265‐275.
18. Onyenmechi JA, Orish EO, Chudi ED, Ejeatuluchukwu OB, Tobias EL. Asomugha and Effects of Rinbacin Extract on Rat Kidney. Biol Pharm. Bull. 2002; 25: 1022‐1025.
19. Swathi D, Sujatha D, Bharathi K, Prasad KV, Antilithiatic activity of the aqueous extract of the roots of Tephrasia purpurea Linn. Pharmacognosy Magazine.2008; 16: 206-211.
20. Vermeulen CW. Experiments on causation of urinary calculi. In: Essays in Experimental Biology. University of Chicago Press, Chicago. 1962; 10(1): 253–269.
21. Prasad KG, Bharathi K, Srinivasan KK. Evaluation of Musa parasidica Linn ‐“Puttubale” stem juice for antilithiatic activity in albino rats. Indian Journal of Physiology and Pharmacology. 1993; 37(4):337–341.
22. Selvam P, Kalaiselvi P, Govindaraj A, Murugan VB, Sathishkumar AS. Effect of A. lanata leaf extract and Vediuppu chunnam on the urinary risk factors of calcium oxalate urolithiasis during experimental hyperoxaluria. Pharmacological Research. 2001; 43(6):89–93.
2. Ibtissam ZR, Younes EL. Lemon juice has protective activity in a rat urolithiasis model. BMC Urol. 2007; 7(2): 18.
3. Christina AJ, Ashok K, Packialakshmi M, Tobin GC, Preethi J, Murugesh N. Antilithiatic effect of Asparagus racemosus willd on Ethylene Glycol‐Indued Lithiasis in Male Albino Wistar Rats. Methods find Experimental clinical pharmacology.2005; 27(3): 633‐638.
4. Aroujo VT, Domingos CD, Silvamonteiro AP, Riggo LimaLandman MT, Lapa AJ, Souccar C. Evaluation of the antiurolithiatic activity of the extract of Costus spiralis Roscoe in rats. Journal of Ethnopharamacology.1999; 66(5): 193-198.
5. Shah GL. Flora of Gujarat Stat. Edn 1, Vol.1, Sardar Patel University, Vallabh Vidyanagar,1978, pp. 543-544.
6. Chattertee A. The Treatise of Indian medicinal plants. Edn 1, Vol.5, National Institute of Scienec Communication and information Resources, New-Delhi, 1987; pp. 63-64.
7. Nadkarni KM. Indian Materia Medica. Popular Prakashan, Bombay, India; 1978.p 667‐669.
8. Kirtikar, Basu BD. Indian Medicinal plants, 2nd ed, 3:Bishen Sing Mahindra Pal Singh Publications;1933.pp 1864‐1865.
9. Mukherjee KP. Quality Control of Herbal Drugs, Edn 1, Business Horizons, New Delhi, India. 2004, pp. 379-380.
10. Kokate CK. Practical Pharmacognosy. Edn4, Vallabh Prakashan, Delhi, India, 1994, pp. 107-113.
11. Evans CW. Trease and Evans Pharmacognosy. Edn 1, Elsevier Ltd, China. 2002, pp. 200-204.
12. Mitra SK, Gopumadhavan G, Venkataranganna MV and Sundaram R. Effect of cystone, a herbal formulation on glycolic acid induced urolithiasis. Phytotherapy research.1998; 12: 372-374.
13. Ravindra VK, Navneet BG, Alagawadi KR, Rudraprabhu VS. Ethylene glycol induced urolithiasis in effect of Moringa oleifera Lam. Root‐wood on rats. Journal of Ethnopharamacology2006; 105(3): 306‐311.
14. Mustafa MA, Medeiros DM. Proximate composition, mineral content and fatty acids of cat fish for different seasons and cooking methods. J Food Sci. 1985; 50: 585‐588.
15. Hodgkinson A, Williams A. An improved colorimetric procedure for urine oxalate. Clin Chim Acta. 1972; 36(2):127‐132.
16. Fiske CH, Subbarow Y. The colorimetric determination of phosphorus. J Biol Chem. 1925; 66:375‐381.
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement using Folia‐ciocalteau reagent. J Biol Chem. 1951; 193(7):265‐275.
18. Onyenmechi JA, Orish EO, Chudi ED, Ejeatuluchukwu OB, Tobias EL. Asomugha and Effects of Rinbacin Extract on Rat Kidney. Biol Pharm. Bull. 2002; 25: 1022‐1025.
19. Swathi D, Sujatha D, Bharathi K, Prasad KV, Antilithiatic activity of the aqueous extract of the roots of Tephrasia purpurea Linn. Pharmacognosy Magazine.2008; 16: 206-211.
20. Vermeulen CW. Experiments on causation of urinary calculi. In: Essays in Experimental Biology. University of Chicago Press, Chicago. 1962; 10(1): 253–269.
21. Prasad KG, Bharathi K, Srinivasan KK. Evaluation of Musa parasidica Linn ‐“Puttubale” stem juice for antilithiatic activity in albino rats. Indian Journal of Physiology and Pharmacology. 1993; 37(4):337–341.
22. Selvam P, Kalaiselvi P, Govindaraj A, Murugan VB, Sathishkumar AS. Effect of A. lanata leaf extract and Vediuppu chunnam on the urinary risk factors of calcium oxalate urolithiasis during experimental hyperoxaluria. Pharmacological Research. 2001; 43(6):89–93.
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01-11-2016
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“ANTI-UROLITHIATIC POTENTIAL OF METHANOLIC EXTRACT OF HYGROPHILA SALICIFOLIA ON ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATSq”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 8, no. 6, Nov. 2016, pp. 287-90, https://doi.org/10.25004/IJPSDR.2016.080602.
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How to Cite
“ANTI-UROLITHIATIC POTENTIAL OF METHANOLIC EXTRACT OF HYGROPHILA SALICIFOLIA ON ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATSq”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 8, no. 6, Nov. 2016, pp. 287-90, https://doi.org/10.25004/IJPSDR.2016.080602.
