DIETARY POLYPHENOLICS SUPPLEMENTATION WITH DRINKING BLACK TEA AMELIORATES GENTAMICIN-INDUCED NEPHROTOXICITY IN MICE
Abstract
Black tea (Camellia sinensis) supplement on renal disorders has poorly been explored. The present study was aimed to identified essential polyphenols present in black tea and it’s the role in gentamicin (GEN) induced nephrotoxicity in mice. The polyphenols present in 2.5% black tea infusion (BT) was determined by HPLC and antioxidant activity was assessed by DPPH radical scavenging. The renoprotective role of BT (125 mg/kg and 250 mg/kg orally for 7 days) was assessed in GEN (80 mg/kg, i.p, daily for 7 days) induced mice. BUN and creatinine was estimated in blood and lipid peroxides, glutathione, catalase and protein was determined in renal tissues. Ten polyphenols including catechin, caffeic acid, rutin, sinapic acid, ferulic acid, p-coumaric acid, myricetin, gallic acid, quercetin and kaempferol were identified and quantified in BT by HPLC. Moreover, it also exhibited powerful DPPH radical scavenging property (IC50 74.75µg/mg black tea). Finally, BT not only significantly and dose dependently (p<0.05) lowered BUN and creatinine in blood and reduced lipid peroxides in kidney, but also eventually enhanced the cellular antioxidants, glutathione and catalase in renal tissues. Therefore, black tea could be a good source of polyphenols that may protect kidneys from gentamicin induced oxidative stress.
Keywords:
Black tea, HPLC, Gentamicin, Nephrotoxicity, Antioxidant, CatechinDOI
https://doi.org/10.25004/IJPSDR.2016.080403References
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14. Surmen-Gur E, Gulten T, Serder Z, Colakogullari M. Chronic black tea administration protects plasma proteins, plasma, liver and kidney lipids against oxidation. Med. Sci. Monit. 2006; 12:102-105.
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17. Sur TK, Hazra AK, Bhattacharyya D, Hazra A. Antiradical and anti-diabetic properties of standardized extract of Sunderban mangrove Rhizophora mucronata. Pharmacognosy Res. 2015; 11: 389-394.
18. Seal T, Choudhury K. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of Bauhinia purpurea and Clerodendrum colebrookianum, wild edible plants of North-Eastern region in India. J. Chem. Pharma. Res. 2015; 7:427-437.
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21. Ali BH, Al Salam S, Al Husseini I, Nemmar A. Comparative protective effect of N-acetyl cysteine and tetramethylpyrazine in rats with gentamicin nephrotoxicity. J. Appl. Toxicol. 2009; 29:302-307.
22. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979; 95: 351-358.
23. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione-s-transferase activities in rat lung and liver. Biochem. Biophys. Acta 1979; 582: 67-78.
24. Sinha AK. Colorimetric assay of catalase. Anal. Biochem. 1972; 47: 389-394.
25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J. Biol. Chem. 1951; 193:265-275.
26. Taber SS, Mueller BA. Drug-associated renal dysfunction. Crit. Care Clin. 2006; 22: 357-374.
27. Vandewalle A, Farman N, Morin JP, Fillastre JP, Hatt PY, Bonvalet JP. Gentamicin incorporation along the nephron: Autoradiographic study on isolated tubules. Kidney Int. 1981; 19:529 539.
28. Li J, Li QX, Xie XF, Ao Y, Tie C, Song R. Differential roles of dihydropyridine calcium antagonist nifedipine, nitrendipine and amlodipine on gentamicin-induced renal tubular toxicity in rats. Eur. J. Pharmacol. 2009; 620: 97-104.
29. Martinez-Salgado C, Eleno N, Tavares P, Rodriguez-Barbero A, Garcia-Criado J, Bolanos JP, Lopez-Novoa JM. Involvement of reactive oxygen species on gentamicin-induced mesangial cell activation. Kidney Int. 2002; 62: 1682-1692.
30. Middleton E, Kandaswami C, Theoharids TC. The effects of plant flavonoids on mammalian cells: implication for inflammation, heart disease and cancer. Pharmacol. Rev. 2000; 52: 673-751.
31. Singh D, Kaur R, Chander V, Chopra K. Antioxidants in the prevention of renal disease. J. Med. Food 2006; 9: 443-450.
32. Buffoli B, Pechanova O, Kojsova S, Andriantsitohaina R, Guigno L, Bianchi R, Rezzani R. Provinol prevents CsA-induced nephrotoxicity by reducing reactive oxygen species, iNOS and NF-kB expression. J. Histochem. Cytochem. 2005; 53: 1459-1468.
33. Yamabe N, Yokozawa T, Oya T, Kim M. Therapeutic potential of (-)-epigallocatechin 3-O-gallate on renal damage in diabetic nephropathy model rats. J. Pharmacol. Exp. Ther. 2006; 319: 228-236.
34. Choi JH, Chai YM, Joo GJ, Rhee IK, Lee IS, Kim KR, Chai MS, Rhee SJ. Effects of green tea catechin on polymorphonuclear leukocyte 5’-lipoxygenase activity, leukotriene B4 synthesis and renal damage in diabetic rats. Ann. Nutr. Metab. 2004; 48:151-155.
2. Crespy V, Williamson GA. Review of the health effects of green tea catechins in in vivo animal models. J. Nutr. 2004; 134: 3431-3440.
3. Dufresne CJ, Farnworth ER. A review of latest research findings on the health promotion properties of tea. J. Nutr. Biochem. 2001; 12: 404-421.
4. Khan N, Mukhtar H. Tea polyphenols for health promotion. Life Sci. 2007; 81:519-33.
5. Gardner EJ, Ruxton CHS, Leeds AR. Black tea - helpful or harmful? A review of the evidence. Eur. J. Clinical Nutri. 2007; 61:3-18.
6. Sharma V, Rao LJ. A thought on the biological activities of black tea. Crit. Rev. Food Sci. Nutr. 2009; 49:379-404.
7. Frei B, Higdon JV. Antioxidant activity of tea polyphenols in vivo: evidence from animal studies. J. Nutr. 2003; 133: 3275-3284.
8. Balakumar P, Rohilla A, Thangathirupathi A. Gentamicin-induced nephrotoxicity: Do we have a promising therapeutic approach to blunt it? Pharmacol. Res. 2010; 62:179-186.
9. Pedraza-Chaveri J, Maldonado PD, Medina-Campos ON, Olivares-Corichi IM, Granados-Silvestre MA, Hernandex-Pando R, Ibarra-Rubio ME. Garlic ameliorates gentamicin nephrotoxicity: relation antioxidant enzymes. Free Radic. Biol. Med. 2000; 29:602-611.
10. Abdel-Naim AB, Abdel-Wahab MH, Attia FF. Protective effects of vitamin E and probucol against gentamicin-induced nephrotoxicity in rats. Pharmacol. Res. 1999; 40: 183-187.
11. Shi S, Zheng SS, Jia CK, Zhu YF, Xie HY. Inhibitory effect of tea polyphenols on transforming growth factor-b1 expression in the rat with cyclosporine A-induced chronic nephrotoxicity. Acta Pharmacol. Sin. 2004; 25: 98-103.
12. Upaganlawar A, Farswan M, Rathod S, Balaraman R. Modification of biochemical parameters of gentamicin nephrotoxicity by coenzyme Q10 and green tea in rats. Indian J. Exp. Biol. 2006; 44: 416-418.
13. Sarkar A, Bhaduri A. Black tea is a powerful chemopreventor of reactive oxygen and nitrogen species: comparison with its individual catechin constituents and green tea. Biochem. Biophys. Res. Commun. 2001; 284: 173-178.
14. Surmen-Gur E, Gulten T, Serder Z, Colakogullari M. Chronic black tea administration protects plasma proteins, plasma, liver and kidney lipids against oxidation. Med. Sci. Monit. 2006; 12:102-105.
15. Committee for the Purpose of Control and Supervision on Experiments on Animals. CPCSEA guidelines for laboratory animal facility. Indian J. Pharmacol. 2003; 35: 257-274.
16. Sur TK, Chatterjee S, Hazra AK, Pradhan R, Chowdhury S. Acute and sub-chronic oral toxicity study of black tea in rodents. Indian J. Pharmacol. 2015; 47: 167-172.
17. Sur TK, Hazra AK, Bhattacharyya D, Hazra A. Antiradical and anti-diabetic properties of standardized extract of Sunderban mangrove Rhizophora mucronata. Pharmacognosy Res. 2015; 11: 389-394.
18. Seal T, Choudhury K. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of Bauhinia purpurea and Clerodendrum colebrookianum, wild edible plants of North-Eastern region in India. J. Chem. Pharma. Res. 2015; 7:427-437.
19. International Conference on Harmonization (ICH). Validation of analytical procedures: text and methodology Q2 (R1). ICH Expert Working Group, 2005.
20. Organization for Economic Co-operation and Development (OECD) Guideline No. 423. Acute oral toxicity in animals. OECD/OCDE No. 423, adopted 17th December, 2001.
21. Ali BH, Al Salam S, Al Husseini I, Nemmar A. Comparative protective effect of N-acetyl cysteine and tetramethylpyrazine in rats with gentamicin nephrotoxicity. J. Appl. Toxicol. 2009; 29:302-307.
22. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979; 95: 351-358.
23. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione-s-transferase activities in rat lung and liver. Biochem. Biophys. Acta 1979; 582: 67-78.
24. Sinha AK. Colorimetric assay of catalase. Anal. Biochem. 1972; 47: 389-394.
25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J. Biol. Chem. 1951; 193:265-275.
26. Taber SS, Mueller BA. Drug-associated renal dysfunction. Crit. Care Clin. 2006; 22: 357-374.
27. Vandewalle A, Farman N, Morin JP, Fillastre JP, Hatt PY, Bonvalet JP. Gentamicin incorporation along the nephron: Autoradiographic study on isolated tubules. Kidney Int. 1981; 19:529 539.
28. Li J, Li QX, Xie XF, Ao Y, Tie C, Song R. Differential roles of dihydropyridine calcium antagonist nifedipine, nitrendipine and amlodipine on gentamicin-induced renal tubular toxicity in rats. Eur. J. Pharmacol. 2009; 620: 97-104.
29. Martinez-Salgado C, Eleno N, Tavares P, Rodriguez-Barbero A, Garcia-Criado J, Bolanos JP, Lopez-Novoa JM. Involvement of reactive oxygen species on gentamicin-induced mesangial cell activation. Kidney Int. 2002; 62: 1682-1692.
30. Middleton E, Kandaswami C, Theoharids TC. The effects of plant flavonoids on mammalian cells: implication for inflammation, heart disease and cancer. Pharmacol. Rev. 2000; 52: 673-751.
31. Singh D, Kaur R, Chander V, Chopra K. Antioxidants in the prevention of renal disease. J. Med. Food 2006; 9: 443-450.
32. Buffoli B, Pechanova O, Kojsova S, Andriantsitohaina R, Guigno L, Bianchi R, Rezzani R. Provinol prevents CsA-induced nephrotoxicity by reducing reactive oxygen species, iNOS and NF-kB expression. J. Histochem. Cytochem. 2005; 53: 1459-1468.
33. Yamabe N, Yokozawa T, Oya T, Kim M. Therapeutic potential of (-)-epigallocatechin 3-O-gallate on renal damage in diabetic nephropathy model rats. J. Pharmacol. Exp. Ther. 2006; 319: 228-236.
34. Choi JH, Chai YM, Joo GJ, Rhee IK, Lee IS, Kim KR, Chai MS, Rhee SJ. Effects of green tea catechin on polymorphonuclear leukocyte 5’-lipoxygenase activity, leukotriene B4 synthesis and renal damage in diabetic rats. Ann. Nutr. Metab. 2004; 48:151-155.
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01-07-2016
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“DIETARY POLYPHENOLICS SUPPLEMENTATION WITH DRINKING BLACK TEA AMELIORATES GENTAMICIN-INDUCED NEPHROTOXICITY IN MICE”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 8, no. 4, July 2016, pp. 204-9, https://doi.org/10.25004/IJPSDR.2016.080403.
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Copyright (c) 2016 A. Adhikari, A. K. Hazra, S. Bhattacharya, T. Seal, T. K. Sur
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
“DIETARY POLYPHENOLICS SUPPLEMENTATION WITH DRINKING BLACK TEA AMELIORATES GENTAMICIN-INDUCED NEPHROTOXICITY IN MICE”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 8, no. 4, July 2016, pp. 204-9, https://doi.org/10.25004/IJPSDR.2016.080403.
