ANTIDIABETIC ACTIVITY OF EXTRACTS OF PITHECELLOBIUM DULCE BENTH LEAVES IN ALLOXAN INDUCED DIABETIC RATS
Abstract
The objective of the present study was to study effect of Pithecellobium dulce Benth (P. dulce) leaves in alloxan induced diabetic rats. The P. dulce leaves were extracted by maceration and soxhelation method by using water and ethanol as solvent. Acute toxicity study was performed according to OECD 425 guidelines for both aqueous and ethanolic extracts of P. dulce leaves. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. Animals were rendered diabetic by administration of alloxan (130 mg/kg, i.p.). The albino rats were divided in to seven groups with five animals in each group. Diabetic animals were treated with aqueous and ethanolic extract for 20 days. Then blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, aspartate aminotransferase (AST), alanine transaminase (ALT) and glycogen level in liver, muscle and kidney were estimated according to standard procedures. The result shows significant decrease in blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, AST and ALT level when compared to diabetic group. The liver and muscle glycogen level was increased significantly in extract treated groups when compared to diabetic control group. Both extract of P. dulce posses antidiabetic and hypolipidemic potential.
Keywords:
Pithecellobium dulce Benth, antidiabetic, alloxan, lipid profile, glycogen, OGTTDOI
https://doi.org/10.25004/IJPSDR.2016.080507References
1. Rang SP, Ritter JM, Flower PJ, Henderson G. Rang and Dales Pharmacology. Edn 08, Elsevier Ltd., New Delhi, 2015, pp. 385-386.
2. Harsh M. Text book of pathology. Edn. 07, Jaypee brothers medical publishers (p) Ltd., 2016, pp. 808-813.
3. Byron JH. Review complications of diabetes mellitus. Int. J. Diab. Dev. Countries. 2005; 25:63-69.
4. WHO, Definition, diagnosis and classification of diabetes mellitus and its complications. Geneva: World Health Organization Department on Non communicable Disease Surveillance, 1999.
5. Alfiani UP, Tanti AS. Antidiabetic activity of durian (duriozibethinus murr.) and rambutan (nephelium lappaceum l.) Fruit peels in alloxan diabetic rats. Procedia Food Science. 2015; 3:255-261.
6. Tripathi KD. Essentials of Medical Pharmacology. Edn 6, Jaypee Brothers Medical Publishers (P) Ltd, New Delhi, 2008, pp. 266-273.
7. Kirtikar KR, Basu BD. Indian medicinal plants. Edn. 02, International Book Distributors, Dehradun, 1975, pp. 946-947.
8. Zapesochnaya GG. Flavonoids of the leaves of Pithecellobium dulce. Khim Prir Soedin. 1980; 2:252.
9. Megala J, Geetha A. Free radical-scavenging and H+, K+-ATPase inhibition activities of Pithecellobium. Food Chemistry. 2010; 121:1120-1128.
10. Khare CP. Indian medicinal plants an illustrated dictionary. Edn 01, New, Springer (India) Private Limited, New Delhi, 2007, pp. 496.
11. Mule VS, Potdar VH, Jadhav SD, Disouza JI. Neuropharmacological profile of aqueous and ethanolic extract of Pithecellobium dulce benth leaves in mice. Research J. Pharmacology and Pharmacodynamics. 2011; 3:27-30.
12. Nguta JM, Appiah-Opong R, Nyarko AK et al. Antimycobacterial and cytotoxic activity of selected medicinal plant extracts. Journal of Ethnopharmacology. 2016; 182:10-15.
13. Rajan M, Thangraj P. Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckia parvifolia Arn, An in vitro approach. Journal of King saud university science. 2014; 26:267-275.
14. Simone AD, Aline EO, Alexandre BF, Jaqueline NP, Patricia P. Neuropharmacological and genotoxic evaluation of ethanol extract from Erythrina falcata leaves, a plant used in Brazilian folk medicine. Brazilian Journal of Pharmacognosy. 2013; 23(2):335-341.
15. Dora MB, Isabel CG, Julieta V, Fernando A, Andrea G, Jose AF. Neuropharmacological effects of the ethanolic extract of Sida acuta. Brazilian Journal of Pharmacognosy. 2016; 26:209-15.
16. Organization for Economic Cooperation and Development (OECD) guidelines for acute toxicity of chemicals. No. 425 (Adopted: 3 October 2008).
17. Onyeso GI, Nkpaa KW, Omenihu S. Co-administration of caffeine and hydromethanolic fraction of Citrullus lanatus seeds improved testicular functions in alloxan-induced diabetic male Wistar rats. Asian Pacific Journal of Reproduction. 2016; 5(2):105-110.
18. Isaak FF, Heather MC, Matthew JQ, Michael DW, Ward WK. Induction of Type-1 Diabetes Mellitus in Laboratory Rats by Use of Alloxan: Route of Administration, Pitfalls, and Insulin Treatment. Comparative Medicine. 2004; 3(54):252-257.
19. Frode TS, Medeiros YS. Animal models to test drugs with potential antidiabetic activity. Journal of Ethnopharmacology. 2008; 115:173-183.
20. Dallaqua B, Saito FH, Rodrigues T et al. Treatment with Azadirachta indica in diabetic pregnant rats: Negative effects on maternal outcome. Journal of Ethnopharmacology. 2012; 143:805-811.
21. Silva MG, Aragão TP, Vasconcelos CF et al. Acute and subacute toxicity of Cassia occidentalis L. stem and leaf in Wistar rats. Journal of Ethnopharmacology. 2011; 136:341-346.
22. Okey AO, Paul CC, Agomuo CO. Blood glucose level and lipid profile of alloxan induced hyperglycemic rats treated with single and combinatorial herbal formulations. Journal of Traditional and Complementary Medicine. 2016; 6:184-192.
23. Szkudelski T. The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Physiol. Res. 2001; 50:536-546.
24. Lenzen S. The mechanisms of alloxan and streptozotocin induced diabetes. Diabetologia. 2008; 51:216-226.
25. Anthoneth NE, Chinna NO, Orish EO. Cytological and biochemical studies during the progression of alloxan induced diabetes and possible protection of an aqueous leaf extract of Costus. Chinese Journal of Natural Medicines. 2014; 12(10):745-752.
26. Bartholomew OI, Maxwell IE. Preliminary study of antidiabetic activity of the methanolic leaf extract of Axonopus compressus (P. Beauv) in alloxan induced diabetic rats. Journal of Ethnopharmacology. 2011; 138(3):713-716.
27. Mahendran S, Badami S, Maithili V. Evaluation of antidiabetic effect of embelin from Embelia ribes in alloxan induced diabetes in rats. Biomedicine & Preventive Nutrition. 2011; 1(1):25-31.
28. Ewenighi CO, Dimkpa U, Adejumo BI et al. Estimation of lipid profile and glucose level in alloxan-induced diabetic rats treated with Cymbopogon citratus (lemongrass). J Exp Integr Med. 2013; 3(3):249-253.
29. Poongothai K, Ponmurugun P, Ahmed KS, Kumar BS, Sherift SA. Antihyperglycemic and antioxidant effects of Solanum xanthocarpum leaves (field grown & in vitro raised) extracts on alloxan induced diabetic rats. Asian Pacific Journal of Tropical Medicine. 2011; 778-785.
30. Mule VS, Naikwade NS, Magdum CS, Jagtap VA. Effect of pithecellobium dulce Benth leaves in dexamethasone induced diabetic rats. International journal of pharmacy and pharmaceutical sciences. 2016; 9(8):317-319.
31. Balamurugan K, Nishanthini A, Mohan VR. Antidiabetic and antihyperlipidaemic activity of ethanol extract of Melastoma malabathricum Linn. leaf in alloxan induced diabetic rats. Asian Pac J Trop Biomed. 2014; 4(1):442-448.
32. Ramachandran S, Rajasekaran A, Kumar KT. Antidiabetic, antihyperlipidemic and antioxidant potential of methanol extract of Tectona grandis flowers in streptozotocin induced diabetic rats. Asian Pacific Journal of Tropical Medicine. 2011; 624-631.
33. Jarald EE, Joshi SB, Jain DC, Edwin S. Biochemical Evaluation of the Hypoglycemic Effects of Extract and Fraction of Cassia fistula Linn. in Alloxan-induced Diabetic Rats. Indian J Pharm Sci. 2013; 75(4):427-434.
34. Manickam D, Periyasami L. Antidiabetic effect of methanolic extract of Decalepis hamiltonii root (wight and Arn) in normal and alloxan induced diabetic rats. Journal of pharmacy research. 2013; 6:166-172.
2. Harsh M. Text book of pathology. Edn. 07, Jaypee brothers medical publishers (p) Ltd., 2016, pp. 808-813.
3. Byron JH. Review complications of diabetes mellitus. Int. J. Diab. Dev. Countries. 2005; 25:63-69.
4. WHO, Definition, diagnosis and classification of diabetes mellitus and its complications. Geneva: World Health Organization Department on Non communicable Disease Surveillance, 1999.
5. Alfiani UP, Tanti AS. Antidiabetic activity of durian (duriozibethinus murr.) and rambutan (nephelium lappaceum l.) Fruit peels in alloxan diabetic rats. Procedia Food Science. 2015; 3:255-261.
6. Tripathi KD. Essentials of Medical Pharmacology. Edn 6, Jaypee Brothers Medical Publishers (P) Ltd, New Delhi, 2008, pp. 266-273.
7. Kirtikar KR, Basu BD. Indian medicinal plants. Edn. 02, International Book Distributors, Dehradun, 1975, pp. 946-947.
8. Zapesochnaya GG. Flavonoids of the leaves of Pithecellobium dulce. Khim Prir Soedin. 1980; 2:252.
9. Megala J, Geetha A. Free radical-scavenging and H+, K+-ATPase inhibition activities of Pithecellobium. Food Chemistry. 2010; 121:1120-1128.
10. Khare CP. Indian medicinal plants an illustrated dictionary. Edn 01, New, Springer (India) Private Limited, New Delhi, 2007, pp. 496.
11. Mule VS, Potdar VH, Jadhav SD, Disouza JI. Neuropharmacological profile of aqueous and ethanolic extract of Pithecellobium dulce benth leaves in mice. Research J. Pharmacology and Pharmacodynamics. 2011; 3:27-30.
12. Nguta JM, Appiah-Opong R, Nyarko AK et al. Antimycobacterial and cytotoxic activity of selected medicinal plant extracts. Journal of Ethnopharmacology. 2016; 182:10-15.
13. Rajan M, Thangraj P. Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckia parvifolia Arn, An in vitro approach. Journal of King saud university science. 2014; 26:267-275.
14. Simone AD, Aline EO, Alexandre BF, Jaqueline NP, Patricia P. Neuropharmacological and genotoxic evaluation of ethanol extract from Erythrina falcata leaves, a plant used in Brazilian folk medicine. Brazilian Journal of Pharmacognosy. 2013; 23(2):335-341.
15. Dora MB, Isabel CG, Julieta V, Fernando A, Andrea G, Jose AF. Neuropharmacological effects of the ethanolic extract of Sida acuta. Brazilian Journal of Pharmacognosy. 2016; 26:209-15.
16. Organization for Economic Cooperation and Development (OECD) guidelines for acute toxicity of chemicals. No. 425 (Adopted: 3 October 2008).
17. Onyeso GI, Nkpaa KW, Omenihu S. Co-administration of caffeine and hydromethanolic fraction of Citrullus lanatus seeds improved testicular functions in alloxan-induced diabetic male Wistar rats. Asian Pacific Journal of Reproduction. 2016; 5(2):105-110.
18. Isaak FF, Heather MC, Matthew JQ, Michael DW, Ward WK. Induction of Type-1 Diabetes Mellitus in Laboratory Rats by Use of Alloxan: Route of Administration, Pitfalls, and Insulin Treatment. Comparative Medicine. 2004; 3(54):252-257.
19. Frode TS, Medeiros YS. Animal models to test drugs with potential antidiabetic activity. Journal of Ethnopharmacology. 2008; 115:173-183.
20. Dallaqua B, Saito FH, Rodrigues T et al. Treatment with Azadirachta indica in diabetic pregnant rats: Negative effects on maternal outcome. Journal of Ethnopharmacology. 2012; 143:805-811.
21. Silva MG, Aragão TP, Vasconcelos CF et al. Acute and subacute toxicity of Cassia occidentalis L. stem and leaf in Wistar rats. Journal of Ethnopharmacology. 2011; 136:341-346.
22. Okey AO, Paul CC, Agomuo CO. Blood glucose level and lipid profile of alloxan induced hyperglycemic rats treated with single and combinatorial herbal formulations. Journal of Traditional and Complementary Medicine. 2016; 6:184-192.
23. Szkudelski T. The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Physiol. Res. 2001; 50:536-546.
24. Lenzen S. The mechanisms of alloxan and streptozotocin induced diabetes. Diabetologia. 2008; 51:216-226.
25. Anthoneth NE, Chinna NO, Orish EO. Cytological and biochemical studies during the progression of alloxan induced diabetes and possible protection of an aqueous leaf extract of Costus. Chinese Journal of Natural Medicines. 2014; 12(10):745-752.
26. Bartholomew OI, Maxwell IE. Preliminary study of antidiabetic activity of the methanolic leaf extract of Axonopus compressus (P. Beauv) in alloxan induced diabetic rats. Journal of Ethnopharmacology. 2011; 138(3):713-716.
27. Mahendran S, Badami S, Maithili V. Evaluation of antidiabetic effect of embelin from Embelia ribes in alloxan induced diabetes in rats. Biomedicine & Preventive Nutrition. 2011; 1(1):25-31.
28. Ewenighi CO, Dimkpa U, Adejumo BI et al. Estimation of lipid profile and glucose level in alloxan-induced diabetic rats treated with Cymbopogon citratus (lemongrass). J Exp Integr Med. 2013; 3(3):249-253.
29. Poongothai K, Ponmurugun P, Ahmed KS, Kumar BS, Sherift SA. Antihyperglycemic and antioxidant effects of Solanum xanthocarpum leaves (field grown & in vitro raised) extracts on alloxan induced diabetic rats. Asian Pacific Journal of Tropical Medicine. 2011; 778-785.
30. Mule VS, Naikwade NS, Magdum CS, Jagtap VA. Effect of pithecellobium dulce Benth leaves in dexamethasone induced diabetic rats. International journal of pharmacy and pharmaceutical sciences. 2016; 9(8):317-319.
31. Balamurugan K, Nishanthini A, Mohan VR. Antidiabetic and antihyperlipidaemic activity of ethanol extract of Melastoma malabathricum Linn. leaf in alloxan induced diabetic rats. Asian Pac J Trop Biomed. 2014; 4(1):442-448.
32. Ramachandran S, Rajasekaran A, Kumar KT. Antidiabetic, antihyperlipidemic and antioxidant potential of methanol extract of Tectona grandis flowers in streptozotocin induced diabetic rats. Asian Pacific Journal of Tropical Medicine. 2011; 624-631.
33. Jarald EE, Joshi SB, Jain DC, Edwin S. Biochemical Evaluation of the Hypoglycemic Effects of Extract and Fraction of Cassia fistula Linn. in Alloxan-induced Diabetic Rats. Indian J Pharm Sci. 2013; 75(4):427-434.
34. Manickam D, Periyasami L. Antidiabetic effect of methanolic extract of Decalepis hamiltonii root (wight and Arn) in normal and alloxan induced diabetic rats. Journal of pharmacy research. 2013; 6:166-172.
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01-09-2016
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“ANTIDIABETIC ACTIVITY OF EXTRACTS OF PITHECELLOBIUM DULCE BENTH LEAVES IN ALLOXAN INDUCED DIABETIC RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 8, no. 5, Sept. 2016, pp. 275-80, https://doi.org/10.25004/IJPSDR.2016.080507.
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How to Cite
“ANTIDIABETIC ACTIVITY OF EXTRACTS OF PITHECELLOBIUM DULCE BENTH LEAVES IN ALLOXAN INDUCED DIABETIC RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 8, no. 5, Sept. 2016, pp. 275-80, https://doi.org/10.25004/IJPSDR.2016.080507.
