AN ASSESSMENT OF VARIATION IN ACTIVE INGREDIENTS OF AMPUCARE FROM DIFFERENT ZONES OF INDIA
Abstract
The present study was designed to assess the variation in curcumin content of Curcuma longa rhizome and total polyphenols in Azadirachta indica bark samples procured from different zones of India. Physico-chemical tests such as total ash, acid insoluble ash, alcohol soluble extractive, water soluble extractive, volatile oil content etc. were also determined. A slight variation was seen in the bark samples of Azadirachta indica. Total ash ranged from 4.25±0.15 (East zone) to 5.57±0.20 (Central zone) whereas acid insoluble ash ranged from 0.80±0.07 (East zone) to 1.52±0.06 (Central zone). Alcohol soluble extractive value of East zone sample was found to be more than 4 times higher 16.95±0.80 than that of central zone 3.85±0.12, where lowest value was recorded. Water soluble extractive value was also found to be highest in East zone sample 17.80±1.10 which was 2 times more than that of Central zone sample 8.45±0.15. This variation may be due to variation in climatic conditions, soil type, pollution stress etc. All the results were within the limits as given in The Ayurvedic Pharmacopoeia of India. Total Polyphenols ranged from 190.0 mgGAE/g of dry weight (Central zone) to 510.0 mgGAE/g of dry weight (East zone). All the samples were found to be rich in total polyphenols. In Curcuma longa samples, Total ash ranged from 3.10±0.20 (South zone) to 4.80±0.25 (North zone) whereas acid insoluble ash ranged from 0.55±0.04 (South zone) to 1.24±0.06 (North zone). Alcohol soluble extractive value was found to be highest in South zone sample 14.80±0.30 which was found to be more than 3 times higher than that of central zone 4.28±0.19 where lowest value was recorded. Water soluble extractive value was also found to be highest in West zone sample 12.55±0.69 and lowest in Central zone sample 8.90±0.37. Volatile oil ranged from 3.50±0.21 North zone sample to 5.50±0.20 South zone sample, where highest volatile oil was recovered. All the results were within the limits as given in The Ayurvedic Pharmacopoeia of India. In our study, curcumin content ranged from 0.30 % (Central zone) to 3.24 % (South zone). Average curcumin content was found to be 1.54 % on dry weight basis. Ampucare is prepared by selection of best quality herbs.
Keywords:
Ampucare, Curcuma longa, Azadirchta indica, Curcumin, Total polyphenols, Total ashDOI
https://doi.org/10.25004/IJPSDR.2010.020208References
2. Wuthi-udomlert M, Grisanapan W, Luanratana O, Caichompoo W. Antifungal activity of Curcuma longa grown in Thailand. Southeast Asian J Trop Med Public Health 2000; 31(1): 178-182.
3. Jain A, Basal E. Inhibition of Propionibacterium acnes-induced mediators of inflammation by Indian herbs. Phytomedicine 2003; 10: 34-38.
4. Singh R, Chandra R, Bose M, Luthra PM. Antibacterial activity of Curcuma longa rhizome extract on pathogenic bacteria. Current Science 2002; 83: 737-740.
5. Goel A, Kunnumakkara AB, Aggarwal BB. Curcumin as "Curecumin": from kitchen to clinic. Biochem Pharmacol 2008 Feb 15; 75(4):787-809.
6. Jayaprakasha GK, Rao LJ, Sakariah KK. Improved HPLC method for the determination of curcumin, demethoxycurcumin and bisdemethoxycurcumin. J Agric Food Chemistry 2002; 50: 3668-3672.
7. Panchatcharam M, Miriyala S, Gayathri VS, Suguna L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol Cell Biochem 2006; 290: 87-96.
8. Thangapazham RL, Sharma A, Maheshwari RK. Beneficial role of curcumin in skin diseases. Adv Exp Med Biol 2007; 595: 343-357.
9. Naithani V. Bark of Indian Medicinal Plants: An Introduction. First edition, Paperlinks publishers, Shahdara, Delhi (India), 2009.
10. Fabry W, Okemo P, Ansorg, R. Activity of east African medicinal plants against Helicobacter pylori. Chemotherapy, 1996a; 42: 315-317.
11. Fabry W, Okemo P, Ansorg R. Fungistatic and fungicidal activity of east African medicinal plants. Mycoses 1996b; 39: 67-70.
12. Wolinsky LE, Mania S, Nachnani S, Ling, S. The inhibiting effect of aqueous Azadirachta indica (Neem) extract upon bacterial properties influencing in vitro plaque formation. J Dent Res., 1996; 75: 816-822.
13. Bandyopadhyay U, Biswas K, Sengupta A, Moitra P, Dutta P, Sarkar D, Debnath P, Ganguly CK, Banerjee RK. Clinical studies on the effect of Neem (Azadirachta indica) bark extract on gastric secretion and gastroduodenal ulcer. Life Sci. 2004; 75: 2867-2878.
14. Subapriya R, Nagini S. Medicinal properties of neem leaves: a review. Curr. Med. Chem. Anticancer Agents 2005; 5: 149-156.
15. Merriam-Webster. http://www.merriam-webster.com/medical/polyphenol. Retrieved on 03/02/10. Merriam-Webster Incorporated, 2010.
16. Scutt AM, Meghji S, Caniff JP, Harvey W. Stabilization of collagen by betel nut polyphenol as a mechanism in oral submucous fibrosis. Experimentia1987; 43:391-393.
17. Klass BR, Branford OA, Grobbelaar AO, Rolfe KJ. The effect of epigallocatechin-3-gallate, a constituent of green tea, on transforming growth factor-beta1-stimulated wound contraction. Wound Repair Regen. 2010; Jan-Feb 18(1):80-8.
18. Shen YJ, Li WG, Zhang H, Li B, Yuan HX, Zhang JJ. Effects of (-)-epigallocatechin-3-gallate on interleukin-1beta expression in mouse wound healing. Fen Zi Xi Bao Sheng Wu Xue Bao. 2009; Jun 42(3-4):179-85.
19. The Ayurvedic Pharmacopoeia of India, Vol. I, Part I, 1st edition, Govt. of India, Ministry of Health & Family Welfare, Dept. of ISM & H, New Delhi, India, 1989.
20. IS 10925. Turmeric oleoresin. Manak Bhavan, 9 Bahadur Shah Zafar Marg, New Delhi India, 1984.
21. Singleton VL, Rossi JA JR. Colorimetry of total phenolics with phosphomolybdic acid-phosphotungstic acid reagents. Am. J. Enol. Viticult. 1965; 16: 144-158.
22. The Ayurvedic Pharmacopoeia of India, Vol. II, Part I, 1st edition, Govt. of India, Ministry of Health & Family Welfare, Dept. of ISM & H, New Delhi, India, 1999.
23. Fu M, Zhou L. Application of plant polyphenols in environmental protection and agricultural production. Ying Yong Sheng Tai Xue Bao, 2004; 15(9): 1673-1677.
24. Gupta A, Kumar R, Upadhyay NK, Pal K, Kumar R, Sawhney RC. Effects of Rhodiola imbricata on dermal wound healing. Planta Med. 2007; 73(8): 774-777.
25. Dulak J. Nutraceuticals as anti-angiogenic agents: hopes and reality. J Physiol Pharmacol. 2005; Mar 56 Suppl 1: 51-67.
26. Madhan B, Subramanian V, Rao JR, Nair BU, Ramasami T. Stabilization of collagen using plant polyphenol: role of catechin. Int J Biol Macromol. 2005; Oct 30, 37(1-2):47-53.
27. Tayyem RF, Health DD, Al-Delaimy WK, Rock CL. Curcumin content of turmeric and curry powders. Nutrition and Cancer 2006; 55: 126-131 (2006).
28. Garg SN, Bansal RP, Gupta MM, Kumar S. Variation in the rhizome essential oil and curcumin contents and oil quality in the land races of turmeric Curcuma longa of North Indian plains. Flavour and Fragrance Journal 1999; 14: 315-318.
29. Li L, Fu S, Qing S. Effect of growth period, storage time and varieties on the contents of main active constituents of Curcuma longa L. in rhizome. Zhongguo Zhong Yao Za Zhi, 1999; 4: 589-590, 637.
30. Tonnesen HH, Karlsen J, Adhikary SR, Pandey. Studies on curcumin and curcuminoids XVII. Variation in the content of curcuminoids in Curcuma longa L. from Nenal during one season. Zeitschrift für Lebensmitteluntersuchung und -Forschung A 1989; Volume 189, Number 2: 116-118.
31. Mudau FN, Soundy ES, Toit DU, Oliver J. Variation in polyphenolic content of Athrixia phylicoides (bush tea) leaves with season and nitrogen application. South African Journal of Botany, 2006; 72: 398-402.
32. Naithani V, Kakkar P. Effect of ecological variation on heavy metal content of some medicinal plants used as herbal ingredients in India. Bull environ Contam Toxicol, 2006; 76: 285-292.
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Copyright (c) 2010 Naveen Pathak, Vijay Naithani, Jasvinder Singh, Parveen Bhole, Manu Chaudhary
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