STABILITY STUDY AND IN-VIVO EVALUATION OF LORNOXICAM LOADED ETHYL CELLULOSE MICROSPHERES
Abstract
Sustained release microspheres of Lornoxicam were prepared by emulsion solvent evaporation method using ethyl cellulose as release retarding agent and evaluated for in-vivo efficacy and stability study. The anti-inflammatory and analgesic activity of microspheres was evaluated by using the carrageenan-induced hind paw edema and Eddy’s hot plate methods, respectively. In-vivo study of microspheres demonstrated significant analgesic and anti-inflammatory activities of prepared microspheres for a longer period of time as compared to the standard drug. The microspheres showed minor changes (non significant) in physical appearance, particle size with no appreciable change in drug content in different conditions of stability studies. Results also showed that no remarkable changes in the drug release from microspheres formulation of different storage conditions. From these findings, it is concluded that Lornoxicam significantly increases anti-inflammatory and analgesic effect when oral administration in ethyl cellulose microspheres formulation.
Keywords:
Lornoxicam, microspheres, solvent evaporation, analgesicDOI
https://doi.org/10.25004/IJPSDR.2014.060104References
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24. Borkar S, Suresh R, Sawant V. An approach to formulate bilayered gastroretentive floating drug delivery system of cefpodoxime proxetil. Int J Chem Tech Res. 2010; 2: 1229-1242.
2. Homdrum EM, Likar R, Nell G. Xefo® rapid: a novel effective tool for pain treatment. Eur Surg. 2006; 38:342-352.
3. Flower RJ, Vane JR. Inhibition of prostaglandin biosynthesis. Biochem Pharmacol. 1974; 23:1439-1450.
4. Skjodt NM, Davies NM. Clinical pharmacokinetics of lornoxicam- A short half-life oxicam. Clinical Pharmacokinetic 1998; 34:421-428.
5. Duarte ARC, Roy C, Vega-González A, Duarte CMM, Subra-Paternault P. Preparation of acetazolamide composite microparticles by supercritical anti-solvent techniques. Int. J Pharm. 2007; 332:132-139.
6. Davis SS, Illum L. Polymeric microspheres as drug carriers. Biomaterials 1988; 9:111-115.
7. Ritschel WA. Biopharmaceutic and pharmacokinetic aspects in the design of controlled release peroral drug delivery systems. Drug Dev Ind Pharm. 1989; 15:1073-1103.
8. Gibaud S, Bonneville A, Astier A. Preparation of 3, 4-diaminopyridine microparticles by solvent-evaporation methods. Int J Pharm. 2002; 242:197-201.
9. Chowdary KPR, Rao NK, Malathi K. Ethyl cellulose microspheres of glipizide: characterization, in vitro and in vivo evaluation. Indian J Pharm Sci. 2004; 66:412-416.
10. Hyppola R, Husson I, Sundholm F. Evaluation of physical properties of plasticized ethyl cellulose films cast from ethanol solution part-I. Int J Pharm. 1996; 133:161-170.
11. Sahoo SK, Mallick AA, Barik BB, Senapati PC. Preparation and in vitro evaluation of ethyl cellulose microspheres containing stavudine by the double emulsion method. Pharmazie 2007; 62:117-121.
12. Wu PC, Huang YB, Chang JI, Tsai MJ, Tsai YH. Preparation and evaluation of sustained release microspheres of potassium chloride prepared with ethyl cellulose. Int J Pharm. 2003; 260:115-121.
13. Atyabi F, Mohammadi A, Dinarvand R. Preparation of nimodipine loaded microspheres: evaluation of parameters. Iran J Pharm Sci. 2005; 1:143-152.
14. Duarte ARC, Costa MS, Simplicio AN, Cardoso MM, Duarte CMM. Preparation of controlled release microspheres using supercritical fluid technology for delivery of anti-inflammatory drugs. Int J Pharm. 2006; 308:168-174.
15. Winter, C.A, E.A. Risley, G.W. Nuss. Carrageenan induced edema in hind paw of the rat as assay for anti-inflammatory drugs. Exp Bio Med. 1962; 111:544-547.
16. Pehlivan B, Cuvas O, Basar H, Bakir F, Ustun H, Dikmen B. Coomparison of the effects of repeated dose treatments of lornoxicam and meloxicam on renal functions in rats. Turkish Journal of Medical Sciences 2012; 40:371-376.
17. Bianchi M, Paneria A. Effects of Lornoxicam, piroxicam and meloxicam in a model of thermal hindpaw hyperalgesia induced by formalin injection in rat trial. Pharma Res. 2002; 45:101-105.
18. Ojewole JAO. Analgesic, anti-inflammatory and hypoglycemic effectscof Rhus chirindensis (Baker F.) [Anacardiaceae] stem-bark aqueous extract in mice and rats, Journal of Ethnopharmacology 2007; 113:338-345.
19. Eddy ND, Leimback D. Synthetic analgesics-II. Dithyienylbutenylamines and dithyienylbutylamines. Journal of Pharmacology and Experimental Therapeutics 1953; 3: 544-547.
20. Matthews BR. Regulatory aspects of stability testing in europe. Drug Dev Ind Pharm. 1999; 25:831-856.
21. Martin A, Bustamante P, Chun AHC. Physical Pharmacy: Physical Chemical Principles in the Pharmaceutical Sciences. Edn 4, B. I. Publications Pvt. Ltd., 2005, pp. 423-448.
22. Soppimath K, Kulkarni A, Aminabhavi T. Encapsulation of antihypertensive drugs in cellulose-based matrix microspheres: characterization and release kinetics of microspheres and tableted microspheres. J. Microencapsulation 2001; 18:397-409.
23. Garud N, Garud A. Preparation and in-vitro evaluation of metformin microspheres using non-aqueous solvent evaporation technique. Trop J Pharm Res. 2012; 11:577-583.
24. Borkar S, Suresh R, Sawant V. An approach to formulate bilayered gastroretentive floating drug delivery system of cefpodoxime proxetil. Int J Chem Tech Res. 2010; 2: 1229-1242.
Published
01-01-2013
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“STABILITY STUDY AND IN-VIVO EVALUATION OF LORNOXICAM LOADED ETHYL CELLULOSE MICROSPHERES”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 1, Jan. 2013, pp. 26-30, https://doi.org/10.25004/IJPSDR.2014.060104.
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How to Cite
“STABILITY STUDY AND IN-VIVO EVALUATION OF LORNOXICAM LOADED ETHYL CELLULOSE MICROSPHERES”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 1, Jan. 2013, pp. 26-30, https://doi.org/10.25004/IJPSDR.2014.060104.
