KINETICS OF ACETYLCHOLINESTERASE INHIBITION BY AN AQUEOUS EXTRACT OF MENTHA LONGIFOLIA LEAVES
Abstract
Cholinesterase inhibitors are the class of compounds which inhibit cholinesterase enzyme. These are used as drugs for symptomatic treatment of Alzheimer’s disease (AD). The present study, evaluate anti-cholinesterase property of an aqueous extract of Mentha longifolia leaves, which is an aromatic plant traditionally used for several medicinal properties. Ellman’s method was used to determine the acetylcholinesterase (AChE) enzyme inhibitory activity of an aqueous extracts of Mentha longifolia leaves which showed concentration dependent AChE inhibition with maximum inhibition of 62.82 ± 0.005% at 25μg/ml final concentrations with IC50 value of 8.004μg/mL. The kinetic study using Lineweaver-Burk plot of an aqueous extract of Mentha longifolia leaves showed mixed non-competitive mode of inhibition against AChE. The anti AChE enzyme activity exhibited by an aqueous extracts of Mentha longifolia leaves extracts might be used in future for symptomatic treatment of AD.
Keywords:
Acetylcholinesterase, Mentha longifolia, Inhibition, Kinetics, Ellman’s method, Alzheimer’s diseaseDOI
https://doi.org/10.25004/IJPSDR.2014.060414References
1. Zilka N, Novak M. The tangled story of Alois Alzheimer. Bratisl Lek Listy. 2006; 107(9-10): 343-345.
2. Lazarczyk MJ, Hof PR, Bouras C, Giannakopoulos P. Preclinical Alzheimer disease: identification of cases at risk among cognitively intact older individuals. BMC Med. 2012; 10:127.
3. Ropper AH, Brown RH. Principles of Neurology. The McGraw Hill Companies, New York 8th edition, 2005.
4. Falchook AD, Mayberry RI, Poizner H, Burtis DB, Doty L, Heilman KM. Sign language aphasia from a neurodegenerative disease. Neurocase. 2012; 19(5): 434-44.
5. Giacobini E. Cholinesterases: new roles in brain function and in Alzheimer's disease. Neurochem Res. 2003; 28 (3-4): 515-522.
6. Weinstock M. Selectivity of cholinesterase inhibition: clinical implications for the treatment of Alzheimer’s disease. CNS Drugs. 1999; 12: 307–323.
7. Sims NR, Bowen DM, Allen SJ, Smith CC, Neary D, Thomas DJ. Presynaptic cholinergic dysfunction in patients with dementia. J Neurochem. 1983; 40: 503–509.
8. Whitehouse PJ. Cholinergic therapy in dementia. Acta Neurologica.1993; 149: 42–45.
9. Nordberg A, Svensson AL. Cholinesterase inhibitors in the treatment of Alzheimers disease (A comparison of tolerability and pharmacology). Drug safety.1998; 19(6): 465-480.
10. Mukherjee PK, Kumar V, Mal M, Houghton PJ, Acetylcholinesterase inhibitors from plants. Phytomedicine 2007; 14: 289–300.
11. Vladimir-Knezevic S, Blazekovic B, Kindl M, Vladic J, Lower-Nedza AD, Brantner AH. Acetylcholinesterase inhibitory, antioxidant and phytochemical properties of selected medicinal plants of the lamiaceae family. Molecules 2014; 19: 767-782.
12. Eissa TAF, Palomino OM, Carretero ME, Gomez-Serranillos. Ethnopharmacological study of medicinal plants used in the treatment of CNS disorders in Sinai Peninsula, Egypt. J Ethnopharmacol. 2014; 151(1): 317-332.
13. Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961; 7: 88-95.
14. Kumar S, Brijeshlata, Dixit S. Screening of traditional Indian spices for inhibitory activity of Acetylcholinesterase and Butyrylcholinesterase enzymes. Int J Pharma Bio Sci. 2012; 3:59-65.
15. Shekhar C, Kumar S. Kinetics of butyrylcholinesterase inhibition by an ethanolic extract of Shorea robusta. Int J Pharma Sci Res. 2014; 5(8): 480-483.
16. Kumar S, Seal CJ, Okello EJ. Kinetics of Acetylcholinesterase inhibition by an aqueous extract of Withania somenifera roots. Int J Pharma Sci Res. 2011; 2(5): 1188-1192.
17. Kumar S, Chowdhary S. Kinetics of acetylcholinesterase inhibition by an aqueous extract of Cuminum cyminum seeds. Int J Appl Sci Biotechnol. 2014; 2(1):64-68.
18. Inglis F. The tolerability and safety of cholinesterase inhibitors in the treatment of dementia. Int J Clin Pract. 2002; 127: 45-63.
19. Mills S, Bone K. Principles and Practice of Phytotherapy: Modern herbal medicine, Edinburgh: Churchill Livingstone, 2000.
20. Lopez V, Martin S, Gomez-Serranillos MP, Carretero ME, Jager AK, Calvo MI. Neuroprotective and neurochemical properties of mint extracts. Phytother Res. 2010; 24(6): 869-874.
21. Bartolini M, Bertucci C, Cavrini V, Andrisano V. Beta-Amyloid aggregation induced by human acetylcholinesterase: inhibition studies. Biochem Pharmacol. 2003; 65: 407-416.
2. Lazarczyk MJ, Hof PR, Bouras C, Giannakopoulos P. Preclinical Alzheimer disease: identification of cases at risk among cognitively intact older individuals. BMC Med. 2012; 10:127.
3. Ropper AH, Brown RH. Principles of Neurology. The McGraw Hill Companies, New York 8th edition, 2005.
4. Falchook AD, Mayberry RI, Poizner H, Burtis DB, Doty L, Heilman KM. Sign language aphasia from a neurodegenerative disease. Neurocase. 2012; 19(5): 434-44.
5. Giacobini E. Cholinesterases: new roles in brain function and in Alzheimer's disease. Neurochem Res. 2003; 28 (3-4): 515-522.
6. Weinstock M. Selectivity of cholinesterase inhibition: clinical implications for the treatment of Alzheimer’s disease. CNS Drugs. 1999; 12: 307–323.
7. Sims NR, Bowen DM, Allen SJ, Smith CC, Neary D, Thomas DJ. Presynaptic cholinergic dysfunction in patients with dementia. J Neurochem. 1983; 40: 503–509.
8. Whitehouse PJ. Cholinergic therapy in dementia. Acta Neurologica.1993; 149: 42–45.
9. Nordberg A, Svensson AL. Cholinesterase inhibitors in the treatment of Alzheimers disease (A comparison of tolerability and pharmacology). Drug safety.1998; 19(6): 465-480.
10. Mukherjee PK, Kumar V, Mal M, Houghton PJ, Acetylcholinesterase inhibitors from plants. Phytomedicine 2007; 14: 289–300.
11. Vladimir-Knezevic S, Blazekovic B, Kindl M, Vladic J, Lower-Nedza AD, Brantner AH. Acetylcholinesterase inhibitory, antioxidant and phytochemical properties of selected medicinal plants of the lamiaceae family. Molecules 2014; 19: 767-782.
12. Eissa TAF, Palomino OM, Carretero ME, Gomez-Serranillos. Ethnopharmacological study of medicinal plants used in the treatment of CNS disorders in Sinai Peninsula, Egypt. J Ethnopharmacol. 2014; 151(1): 317-332.
13. Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961; 7: 88-95.
14. Kumar S, Brijeshlata, Dixit S. Screening of traditional Indian spices for inhibitory activity of Acetylcholinesterase and Butyrylcholinesterase enzymes. Int J Pharma Bio Sci. 2012; 3:59-65.
15. Shekhar C, Kumar S. Kinetics of butyrylcholinesterase inhibition by an ethanolic extract of Shorea robusta. Int J Pharma Sci Res. 2014; 5(8): 480-483.
16. Kumar S, Seal CJ, Okello EJ. Kinetics of Acetylcholinesterase inhibition by an aqueous extract of Withania somenifera roots. Int J Pharma Sci Res. 2011; 2(5): 1188-1192.
17. Kumar S, Chowdhary S. Kinetics of acetylcholinesterase inhibition by an aqueous extract of Cuminum cyminum seeds. Int J Appl Sci Biotechnol. 2014; 2(1):64-68.
18. Inglis F. The tolerability and safety of cholinesterase inhibitors in the treatment of dementia. Int J Clin Pract. 2002; 127: 45-63.
19. Mills S, Bone K. Principles and Practice of Phytotherapy: Modern herbal medicine, Edinburgh: Churchill Livingstone, 2000.
20. Lopez V, Martin S, Gomez-Serranillos MP, Carretero ME, Jager AK, Calvo MI. Neuroprotective and neurochemical properties of mint extracts. Phytother Res. 2010; 24(6): 869-874.
21. Bartolini M, Bertucci C, Cavrini V, Andrisano V. Beta-Amyloid aggregation induced by human acetylcholinesterase: inhibition studies. Biochem Pharmacol. 2003; 65: 407-416.
Published
01-10-2014
Statistics
Abstract Display: 221 
PDF Downloads: 254
Dimension Badge
How to Cite
“KINETICS OF ACETYLCHOLINESTERASE INHIBITION BY AN AQUEOUS EXTRACT OF MENTHA LONGIFOLIA LEAVES”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 4, Oct. 2014, pp. 348-50, https://doi.org/10.25004/IJPSDR.2014.060414.
Issue
Section
Research Article
How to Cite
“KINETICS OF ACETYLCHOLINESTERASE INHIBITION BY AN AQUEOUS EXTRACT OF MENTHA LONGIFOLIA LEAVES”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 4, Oct. 2014, pp. 348-50, https://doi.org/10.25004/IJPSDR.2014.060414.
